FAN Lingyun, HU Zhihong, Just．M．Vlak and CHEN Xinwen. Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene[J]. Virologica Sinica, 2001, 16(4): 344-349.
Citation:
FAN Lingyun, HU Zhihong, Just．M．Vlak, CHEN Xinwen.
Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene .VIROLOGICA SINICA, 2001, 16(4)
: 344-349.
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摘要
对棉铃虫单核衣壳核多角体病毒 (Helicoverpaarmigerasingle -nucleocapsidnucleopolyhedrovirus,HaSNPV)基因组中EcoRI N片段进行序列分析 ,获得了完整的解螺旋酶基因 (hel) ,其开放阅读框大小为 376 2bp ,编码一个分子量为 14 6kD的蛋白质。在hel起始密码子ATG上游 5 0位有强晚期启动子转录起始信号ATAAG ,在 112位和 189位存在两个TATAbox ,但未发现早期转录信号CAGT。其在终止密码子下游第 12位有一PolyA终止信号AATAAA。在其它真核或原核解螺旋酶中存在的 7个保守基元 (I、Ia、II、III、Ⅳ、Ⅴ、Ⅵ) ,只有 5个 (I、Ia、II、III、Ⅳ)在杆状病毒中保守同源性比较发现 ,HaSNPV解螺旋酶的氨基酸序列与甜菜夜蛾核多角体病毒 (SpodopteraexigueMNPV ,SeMNPV)的解螺旋酶具有最高的同源性 (6 6 % ) ,与Xestiac nigrum颗粒体病毒 (XcGV)解螺旋酶的同源性最低 (43% )。HaSNPV解螺旋酶基因是第一个报道的单粒包埋核多角体病毒的解螺旋酶基因 In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8～59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular mass of 146kDa.A late transcriptional motif,ATAAG,was found at 50nt upstream of the translational start codon ATG,while two TATA box was located at 112nt and 189nt upstream of ATG.A typical poly(A) signal was found at 12nt downstream of the translational stop codon.Compared HaSNPV helicase with the helicases of Autographa colifornica MNPV(AcMNPV),( Bombyx bori NPV (BmNPV), Lymantria dispar MNPV(LdMNPV) Spodoptera exigue MNPV (SeMNPV), Orgyia pseudotsugata MNPV (OpMNPV),and Xestia c nigrum granuloviurs(XcGV),only 5motifs(I,Ia,II,III,IV)were found conserved in baculovirus.The homology of the other two notifs (V and VI),which are also quite conserved in other helicases,were lowe
Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene
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Abstract
In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8～59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular mass of 146kDa.A late transcriptional motif,ATAAG,was found at 50nt upstream of the translational start codon ATG,while two TATA box was located at 112nt and 189nt upstream of ATG.A typical poly(A) signal was found at 12nt downstream of the translational stop codon.Compared HaSNPV helicase with the helicases of Autographa colifornica MNPV(AcMNPV),( Bombyx bori NPV (BmNPV), Lymantria dispar MNPV(LdMNPV) Spodoptera exigue MNPV (SeMNPV), Orgyia pseudotsugata MNPV (OpMNPV),and Xestia c nigrum granuloviurs(XcGV),only 5motifs(I,Ia,II,III,IV)were found conserved in baculovirus.The homology of the other two notifs (V and VI),which are also quite conserved in other helicases,were lowe
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