Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus

ZHOU Bin．CHAO Rui—bing; LU Yin—hua; CHEN De—sheng．CHEN Pu-yang*

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April 2003

ZHOU Bin．CHAO Rui—bing, LU Yin—hua, CHEN De—sheng．CHEN Pu-yang*, Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus[J]. Virologica Sinica, 2003, 18(2): 137-140.

Citation: ZHOU Bin．CHAO Rui—bing, LU Yin—hua, CHEN De—sheng．CHEN Pu-yang*, . Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus .VIROLOGICA SINICA, 2003, 18(2) : 137-140.

随机引物PCR扩增鸡胚致死孤儿病毒DNA 的研究

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京．210095

摘要

摘要：在电镜下观察鸭病毒性肝炎病毒鸡胚尿囊液种毒时，另看到一大小为70～80nm，无囊膜，20面体对称的病毒颗粒。为了解该污染病毒，作者挑选了4条随机引物对此未知病毒分别进行反转录PCR和直接PCR扩增，结果共扩增出3条基因片段，经测序(一个反应)后分析与禽腺病毒1型—— 鸡胚致死孤儿病毒基因组部分序列同源性分别高达99．5％、99．6％和99．5％。从而得知鸭病毒性肝炎病毒鸡胚尿囊液种毒受到了鸡胚致死孤儿病毒的严重污染。
- 随机引物
- , PCR
- , 鸡胚致死孤儿病毒

Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus

1.
Key Laboratory of Animal Disease Diagnostic＆ Immunology,Ministry of Agriculture ofPeople’Republic of China， NanjingAgricultural University,Nanjing 210095，China

Abstract

While observing Duck viral hepatitis seed virus in chicken embryonic allantochoroin liquid． another symmetry nonenveloped icosahedral viron with a diam eter of 7O tO 80 am was found．In order to investigation this contam inating virus，we selected four random primers to am plify this unknown virus by RT—PCR method an d PCR method respectively,that resulted three DNA fragments，showing 99．5％，99．6％ an d 99．5％ homologue respectively with the genome of Adenovirus type I，or Chicken embryo orphan lethal virus(CELOV)during the sequencing analysis(one cycle reaction)for them． This results suggested that Duck viral hepatitis seed virus was contam inated by CELOV
- Random primer
- , PCR：Chicken embryo lethal orphan virus (CEL0V)

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus

1. Key Laboratory of Animal Disease Diagnostic＆ Immunology,Ministry of Agriculture ofPeople’Republic of China， NanjingAgricultural University,Nanjing 210095，China

Abstract: While observing Duck viral hepatitis seed virus in chicken embryonic allantochoroin liquid． another symmetry nonenveloped icosahedral viron with a diam eter of 7O tO 80 am was found．In order to investigation this contam inating virus，we selected four random primers to am plify this unknown virus by RT—PCR method an d PCR method respectively,that resulted three DNA fragments，showing 99．5％，99．6％ an d 99．5％ homologue respectively with the genome of Adenovirus type I，or Chicken embryo orphan lethal virus(CELOV)during the sequencing analysis(one cycle reaction)for them． This results suggested that Duck viral hepatitis seed virus was contam inated by CELOV