Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells

GU Chao-jiang; ZHENG Cong—yi; WU Ji—bin; QU San—fu; YANG Wei; CHANG Hui—yun

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October 2003

GU Chao-jiang, ZHENG Cong—yi, WU Ji—bin, QU San—fu, YANG Wei and CHANG Hui—yun. Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells[J]. Virologica Sinica, 2003, 18(5): 459-463.

Citation: GU Chao-jiang, ZHENG Cong—yi, WU Ji—bin, QU San—fu, YANG Wei, CHANG Hui—yun. Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells .VIROLOGICA SINICA, 2003, 18(5) : 459-463.

口蹄疫病毒持续感染细胞系的快速选择及其特性研究

1.
武汉大学生命科学学院，湖北武汉，430072：2．中国农业科学院兰州兽医研究所．甘肃兰州，730046

摘要

摘要：采用NH CI弱碱法处理感染口蹄疫病毒的叙利亚仓鼠肾细胞系(BHK一21)，存活细胞经过单克隆和选择，获得32株阳性克隆细胞株。随机选取一株(BHK—ROp)，常规传代并采用RT-PCR，透射电子显微镜，流式细胞仪进行持续感染特性分析，结果表明，BHK—ROp第4，l6，36代细胞中病毒持续存在，但不影响细胞的生长特性，表现出病毒持续感染的基本特征。可见，NHaCI弱碱法用于建立病毒持续感染细胞系是十分有效的
- 口蹄疫病毒：NH CI法
- , 快速选择：持续感染

Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells

1.
College of Life Sciences，Wuhan University，430072，China

Abstract

Abstract：A baby hamster kidney cell line(BHK一21) infected by foot—and-mouth disease virus (OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32 positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— tured as usual and an alysed with RT_PCR．transmission electron microscope an d flow cytometry．The results demonstrated that the 4th，1 6th，36th generation BHK—ROp cell still harboured ⅥDV which had no chan ges on the growth features of the cells．They had the radical characteristics of viral persistent infection．As noted above，the NH4Cl weak base method used for establishment of the cell line persistently infected with virus was particularly effective．
- Foot-and-mollth disease virus
- , Ammonium chloride method

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Rapid Selection of the Cells Persistently Infected with Foot-and-mouth disease virus and the Study of Natures in Persistent Infection Cells

1. College of Life Sciences，Wuhan University，430072，China

Abstract: Abstract：A baby hamster kidney cell line(BHK一21) infected by foot—and-mouth disease virus (OGBF1 5 strain)was treated with NH4Cl，then the cells survived were selected and cloned．32 positive—cloned cell strains were obtained．One strain fBHK—ROp)was randomly selected and subcul— tured as usual and an alysed with RT_PCR．transmission electron microscope an d flow cytometry．The results demonstrated that the 4th，1 6th，36th generation BHK—ROp cell still harboured ⅥDV which had no chan ges on the growth features of the cells．They had the radical characteristics of viral persistent infection．As noted above，the NH4Cl weak base method used for establishment of the cell line persistently infected with virus was particularly effective．