Immunological Characteristics of a 27kDa Protein in the Bovine Sera

SHAO Xian-an; SHEN Yan; XU Wei; WANG Ying; HU Xin-mei; XIONG Si-dong*

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February 2004

SHAO Xian-an, SHEN Yan, XU Wei, WANG Ying, HU Xin-mei and XIONG Si-dong*. Immunological Characteristics of a 27kDa Protein in the Bovine Sera[J]. Virologica Sinica, 2004, 19(1): 6-9.

Citation: SHAO Xian-an, SHEN Yan, XU Wei, WANG Ying, HU Xin-mei, XIONG Si-dong*. Immunological Characteristics of a 27kDa Protein in the Bovine Sera .VIROLOGICA SINICA, 2004, 19(1) : 6-9.

牛血清中27 kDa蛋白的免疫学特性研究*

1.
复旦大学上海医学院免疫学系，教育部分子医学重点实验室, 上海基因免疫与疫苗研究中心上海 200032

摘要

为探讨牛血清中存在的HBsAg样蛋白的性质，给HBV的发病机制、治疗、预防等研究提供依据，我们采集93份牛血清，以SDS-PAGE、Western Blot对血清中HBsAg样蛋白进行研究，发现其分子量约为27kDa；以SDS-PAGE分离牛血清中的HBsAg样蛋白，经皮下多点免疫小鼠，可产生与人HBV编码蛋白HBsAg反应的抗体。表明该27kDa蛋白可能存在与HBsAg相似的免疫学特性。
- 牛血清
- , 鉴定
- , HBsAg
- , Western Blot
- , 抗原性

Immunological Characteristics of a 27kDa Protein in the Bovine Sera

1.
Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College, Fudan University

Abstract

To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.
- Bovine sera
- , Identification
- , HBsAg
- , Western Blot
- , Antigenicity

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Immunological Characteristics of a 27kDa Protein in the Bovine Sera

1. Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College, Fudan University

Abstract: To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.