A New Gene Subtgpe of BVDV-1 from ZM-95,a Pig-isdate Strain
Abstract: Two earlier Chinese isolates of bovine viral diarrhea virus , bovine CC-184 and pig ZM-95 were phylogenetically analyzed. The E2 gene encoding a major antigenic was obtained by reverse transcription polymerase chain reaction (RT-PCR) and nest-PCR. Sequencing results showed that the E2 genes of CC-184 and ZM-95 are 1122 and 1125 nucleotides in length, encoding 374 and 375 amino acids, respectively. Sequence comparison and phylogenetic analyses demonstrated that both isolates fall into the BVDV-1 group and distant from other Pestiviruses such as BVDV-2, Border disease virus (BDV) and Classical swine fever virus (CSFV). The E2 gene of CC-184 had the highest identity (91.8%) with its homologue in the Osloss strain. However, the E2 gene of ZM-95 is significantly divergent from other known Pestiviruses, with the highest sequence identity of 72. 4% to strain Oregon c24v. Furthermore, isolate ZM-95 addressed significant evolution distance compared with the other established subgroups of BVDV-1, as indicated by phylog