In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus

HUANG Xian-qing; BIE Xiao-mei; LU Zhao-xin; LV Feng-xia; YANG Shu-jing

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October 2006

HUANG Xian-qing, BIE Xiao-mei, LU Zhao-xin, LV Feng-xia and YANG Shu-jing. In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus[J]. Virologica Sinica, 2006, 21(5): 426-429.

Citation: HUANG Xian-qing, BIE Xiao-mei, LU Zhao-xin, LV Feng-xia, YANG Shu-jing. In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus .VIROLOGICA SINICA, 2006, 21(5) : 426-429.

抗微生物脂肽体外抗PRV和PPV的研究*

黄现青 ^1,2 ,
别小妹 ² ,
陆兆新 ^1* ,
吕凤霞 ¹ ,
杨淑晶 ²

1.
1. 南京农业大学食品科技学院，江苏南京，210095
2.
河南农业大学食品科技学院，河南郑州，450002

摘要

本试验研究了枯草芽孢杆菌fmbJ株产生的抗微生物脂肽(Antimicrobial lipopeptide，AMI)的体外抗伪狂犬病病毒(Pseudorabies virus，PRV)、猪细小病毒(Porcine parvovirus, PPV)南京株活性并对其可能的机理进行了初步探讨。结果表明该抗微生物脂肽对猪肾(Porcine Kidney，PK-15)细胞的半数中毒浓度（Median Toxicosis Dose，TD50）和最大无毒浓度（TD0）分别为47.57 mg/L、18.9 mg/L；对PRV株、PPV南京株所致细胞病变效应(Cytopathic Effects，CPE)有明显的抑制作用，可使细胞存活率显著升高；但不能抑制PRV株、PPV南京株在PK-15细胞上的感染和复制。由此可知，该抗微生物脂肽可以直接作用于PRV株、PPV南京株，从而抑制其对PK-15细胞的感染作用，其作用效果显著低于抗病毒药物阿昔洛韦(Acyclovir，ACV)，但由于其对PK-15细胞毒性较弱，可作为一种抗病毒药物进行进一步开发研究。
- 抗微生物脂肽
- , PRV
- , PPV
- , 抗病毒作用

In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus

1.
1.College of Food Science and Technology, Nanjing Agricultural University，Nanjing 210095, China
2.
College of Food schence and Technology, Henan Agricultural University, Zheng zhou 450002, China

Abstract

The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L, respectively. AMI strongly inhibited the cytopathic effect induced by PRV and PPV, and increase the survival rate of cell remarkably. However, it did not inhibit infection and multiplication of PPV and PRV in PK-15 cells. This study indicated that the antimicrobial lipopeptide could affect PRV and PPV directly but precluded infection of PK-15 cells. the effect of AMI in PK-15 cells was markedly lower than the antiviral drug Acyclovir (ACV). Since it had lower toxicity to PK-15 cell, we would study it further as an antiviral drug.
- Antimicrobial lipopeptide
- , Pseudorabies virus
- , Porcine parvovirus
- , Antiviral effect

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

In vitro Assays of an Antimicrobial Lipopeptide Produced by Bacillus subtilis fmbJ Against Pseudorabies Virus and Porcine Parvovirus

1. 1.College of Food Science and Technology, Nanjing Agricultural University，Nanjing 210095, China
2. College of Food schence and Technology, Henan Agricultural University, Zheng zhou 450002, China

Abstract: The effect and mechanism of antimicrobial lipopeptide (AMI) produced by a Bacillus subtilis fmbJ strain against Pseudorabies virus (PRV) and Porcine parvovirus (PPV) were studied. The Median Toxicosis Dose (TD50) and TD0 values of AMI on Porcine Kidney (PK-15) cells were 47.57mg/L and 18.9 mg/L, respectively. AMI strongly inhibited the cytopathic effect induced by PRV and PPV, and increase the survival rate of cell remarkably. However, it did not inhibit infection and multiplication of PPV and PRV in PK-15 cells. This study indicated that the antimicrobial lipopeptide could affect PRV and PPV directly but precluded infection of PK-15 cells. the effect of AMI in PK-15 cells was markedly lower than the antiviral drug Acyclovir (ACV). Since it had lower toxicity to PK-15 cell, we would study it further as an antiviral drug.