Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

MENG Fan-lei; DIAO You-xiang*; WANG Hui; LIU Fang-na; WANG Ni

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October 2006

MENG Fan-lei, DIAO You-xiang*, WANG Hui, LIU Fang-na and WANG Ni. Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus[J]. Virologica Sinica, 2006, 21(5): 468-471.

Citation: MENG Fan-lei, DIAO You-xiang*, WANG Hui, LIU Fang-na, WANG Ni. Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus .VIROLOGICA SINICA, 2006, 21(5) : 468-471.

鸡传染性支气管炎病毒变异株（793/B）核蛋白基因的克隆与序列分析

1.
山东农业大学动物科技学院, 山东泰安271018, China

摘要

根据GenBank已经发表的传染性支气管炎病毒（IBV）N全基因组序列设计引物，对IBV 793/B分离毒株N基因进行克隆与序列分析。结果表明，IBV 793/B的N基因由1229bp组成，与GenBank已发表的11株IBV的N基因相比较，IBV 793/B的N基因共有88处点突变，在第991位发生了一个核苷酸的缺失。N基因的核苷酸同源性为86.9％～91.4%，氨基酸同源性为75.8％～77.5%。表明IBV 93/B的N基因存在着较大的变异性。
- 传染性支气管炎病毒793/B
- , N基因
- , 序列分析

Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

1.
College of Animal Science and Technology, Shandong Agricultural University, Tai,an 271018 China

Abstract

A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9％~91.4％, and the identity of the deduced amino acids was 75.8％~77.5％. The results showed that there were major variatons in the N gene of 793/B.
- 793/B strain of Infectious bronchitis virus
- , Nucleocapsid gene
- , Sequence analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

1. College of Animal Science and Technology, Shandong Agricultural University, Tai,an 271018 China

Abstract: A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9％~91.4％, and the identity of the deduced amino acids was 75.8％~77.5％. The results showed that there were major variatons in the N gene of 793/B.