Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

JI Xiang-bo; LIU Wen-bo; WEI Jian-chao; NIU Ming-fu; ZHOU-bin; CAO Rui-bing; HEN Pu-yan*

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October 2006

JI Xiang-bo, LIU Wen-bo, WEI Jian-chao, NIU Ming-fu, ZHOU-bin, CAO Rui-bing and HEN Pu-yan*. Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene[J]. Virologica Sinica, 2006, 21(5): 481-484.

Citation: JI Xiang-bo, LIU Wen-bo, WEI Jian-chao, NIU Ming-fu, ZHOU-bin, CAO Rui-bing, HEN Pu-yan*. Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene .VIROLOGICA SINICA, 2006, 21(5) : 481-484.

鸡传染性喉气管炎病毒gB基因重组DNA 疫苗的构建与免疫试验

1.
南京农业大学农业部动物疫病诊断与免疫重点开放实验室，江苏南京210095

摘要

本研究构建了编码ILTV主要抗原gB基因的重组DNA疫苗pcDNA-gB，质粒转染293-T细胞的间接免疫表明其表达的蛋白具有免疫反应性。为测定该DNA疫苗的免疫效果，将其与本室保存的重组鸡痘病毒rFPV-gB-gD-IgY分别以单独和混合的方式给4周龄非免疫鸡进行免疫，然后测定ILTV特异性抗体和T淋巴细胞增殖反应。结果表明这2种基因工程疫苗均能诱导鸡产生特异性的体液免疫及细胞免疫应答。其中以pcDNA-gB/rFPV-gB-gD-IgY联合免疫组的效果最好，其诱导的抗体水平已接近于常规弱毒疫苗，而细胞免疫水平则比后者高得多。上述研究结果为ILTV 新型疫苗的研究奠定了基础。
- 传染性喉气管炎
- , DNA疫苗
- , 糖蛋白B
- , 重组鸡痘病毒
- , 免疫效果

Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

1.
Key laboratory of animal disease diagnostic and immunology, Ministry of agricultural science, Nanjing Agricultural University, Nanjing 210095

Abstract

A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation．The study lays foundation on further investigation for the new type of vaccines against ILTV.
- Infectious Laryngotracheitis Virus ( ILTV)
- , DNA Vaccine
- , gB
- , Recombinant fowlpox
- , Immunity

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction and Immune Effect of the Recombinant DNA Vaccine Expressing ILTV Major Antigen Epitope gene

1. Key laboratory of animal disease diagnostic and immunology, Ministry of agricultural science, Nanjing Agricultural University, Nanjing 210095

Abstract: A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus(ILTV) Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells. To evaluate the prophylactic efficacy of the DNA vaccine, 4-week-old chickens were inoculated with pcDNA-gB plasmid and rFPV- gB-gD-IgY, respectively followed by a booster of rFPV-gB-gD-IgY and pcDNA-gB. Anti-ILTV antibodies and T cell proliferation were detected. The results indicated that both vaccines could elicit specific humeral immune and cellular immune responses. Compared with attenuated vaccines, rFPV-gB-gD-IgY/pcDNA-gB elicited not only similar antibody level of ILTV but stronger T cell proliferation．The study lays foundation on further investigation for the new type of vaccines against ILTV.