Genomic Sequencing of S1 Strain of a Porcine Reproductive and  Respiratory Syndrome Virus Isolate

LI Yu-feng; JIANG Ping*; XU Jia-rong; JIANG Wen-ming

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October 2006

LI Yu-feng, JIANG Ping*, XU Jia-rong and JIANG Wen-ming. Genomic Sequencing of S1 Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate[J]. Virologica Sinica, 2006, 21(5): 490-493.

Citation: LI Yu-feng, JIANG Ping*, XU Jia-rong, JIANG Wen-ming. Genomic Sequencing of S1 Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate .VIROLOGICA SINICA, 2006, 21(5) : 490-493.

猪繁殖与呼吸综合征病毒S1株基因组序列测定和分析

1.
南京农业大学动物医学院，江苏南京 210095

摘要

应用RT-PCR方法分段扩增出PRRSV上海分离株S1毒株的4条基因大片段，扩增后的产物分别克隆于pCR-XL-TOPO载体鉴定后测序，同时应用RACE方法对S1毒株的3′和5′ 基因末端进行了成功的扩增并克隆于pMD-18T载体进行测序，按顺序将这些序列进行拼接得到PRRSV S1株全基因组cDNA序列。测序结果表明PRRSV S1株基因组全长15441 bp，包含9个开放式阅读框，5′UTR含有189nt，3′端UTR含有181nt，其中包含30nt Poly (A)。基因组序列分析结果显示该病毒与ATCC VR-2332和BJ-4分离株的核苷酸同源性分别99.5%和99.6%。与另一国内分离株CH-1a的核苷酸同源性为90.8%。
- PRRSV
- , 基因组
- , 序列测定和分析

Genomic Sequencing of S1 Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate

1.
College of Animal Medicine, Nanjing Agricultural University, Nanjing 210095, China

Abstract

Four gene fragments of S1 strain of porcine reproductive and respiratory syndrome virus were amplified by RT-PCR and cloned into the pCR-XL-TOPO vector for sequencing. Authentic 5′ and 3′ terminal fragments of S1 strain were obtained by 5′ -Full RACE Core Set and 3′ -Full RACE Core Set and cloned into the pMD-18T vector and sequenced. The complete nucleotide sequence of S1 strain genome was obtained by sequential splicing of each fragment. The results showed that the genome of S1 strain was 15441 bp in length, containing nine open reading frames (ORFs), with 189 nt in the 5’ UTR and 181 nt in the 3’ UTR, including 30 nt poly (A) tail. The genome of PRRSV S1 strain showed 99.5%, 99.6% and 90.8% of nucleotide identity with ATCC VR-2332, BJ-4 and CH-1a, respectively.
- PRRSV
- , Genome
- , Sequencing and analysis

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Genomic Sequencing of S1 Strain of a Porcine Reproductive and Respiratory Syndrome Virus Isolate

1. College of Animal Medicine, Nanjing Agricultural University, Nanjing 210095, China

Abstract: Four gene fragments of S1 strain of porcine reproductive and respiratory syndrome virus were amplified by RT-PCR and cloned into the pCR-XL-TOPO vector for sequencing. Authentic 5′ and 3′ terminal fragments of S1 strain were obtained by 5′ -Full RACE Core Set and 3′ -Full RACE Core Set and cloned into the pMD-18T vector and sequenced. The complete nucleotide sequence of S1 strain genome was obtained by sequential splicing of each fragment. The results showed that the genome of S1 strain was 15441 bp in length, containing nine open reading frames (ORFs), with 189 nt in the 5’ UTR and 181 nt in the 3’ UTR, including 30 nt poly (A) tail. The genome of PRRSV S1 strain showed 99.5%, 99.6% and 90.8% of nucleotide identity with ATCC VR-2332, BJ-4 and CH-1a, respectively.