采用NCBI提供的BLAST软件在线检索SeMNPV特异性基因ORF22、ORF40，并将其构建到pMD18-T载体上。以纯化的pMD18-T-ORF22、pMD18-T-ORF40质粒为标准样品，已计数野生型SeMNPV的基因组作对照，用荧光定量PCR检测样品中SeMNPV多角体含量。荧光定量PCR检测得到标准曲线方程为con=10(-0.282CT+9.965) （相关系数：R2=0.9997）和con=10(-0.296CT+9.945) (相关系数：R2＝0.9995)。测得一个SeMNPV多角体包含102核酸分子，检测到SeMNPV复合剂包含 633 PIBs/mg、691 PIBs/mg SeMNPV多角体，两者结果一致。与复合剂中SeMNPV实际含量相符。

With the software BLAST from NCBI , the unique gene ORF22 and gene ORF40 of Spodoptera exigua nuclear polyhedrovirus (SeMNPV) were selected and cloned into the pMD18-T vector. The purified pMD18-T-ORF22 and pMD18-T-ORF40 were used as standard samples, genome of quantified SeMNPV were used as control, a real-time TaqMan-based PCR assay was developed to rapidly detect valid quantity of SeMNPV. The equation of standard curves were con=10(-0.282CT+9.965) （R2=0.9997）and con=10(-0.296CT+9.945) (R2=0.9995. This study indicated that a SeMNPV polyhedrobody contained 102 nucleic molecules. SeMNPV complex as detection sample contained 633 PIBs/mg、691 PIBs/mg SeMNPV polyhedrobodies, the two methods were in accordance with each other, and was in accordance with the actual quantity in the samples.