Detection of Human Endogenous Retrovirus env Gene in the Blood of Individuals with Schizophrenia by Using Nested RT-PCR

XIAO Ying; HUANG Wen-jie; LIU Zhong-chun; ZHU Fan; WU Jian-guo

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October 2006

XIAO Ying, HUANG Wen-jie, LIU Zhong-chun, ZHU Fan and WU Jian-guo. Detection of Human Endogenous Retrovirus env Gene in the Blood of Individuals with Schizophrenia by Using Nested RT-PCR[J]. Virologica Sinica, 2006, 21(5): 510-512.

Citation: XIAO Ying, HUANG Wen-jie, LIU Zhong-chun, ZHU Fan, WU Jian-guo. Detection of Human Endogenous Retrovirus env Gene in the Blood of Individuals with Schizophrenia by Using Nested RT-PCR .VIROLOGICA SINICA, 2006, 21(5) : 510-512.

用巢式RT-PCR检测精神分裂症患者血液标本中HERV env基因

肖颖 ¹ ,
黄文杰 ¹ ,
刘忠纯 ² ,
朱帆 ¹ ,
吴建国 ^1*

1.
1.武汉大学生命科学学院病毒学国家重点实验室，湖北武汉430072
2.
武汉大学人民医院，湖北武汉 430060

摘要
- 人内源性逆转录病毒（HERV）
- , 精神分裂症
- , RT-PCR

Detection of Human Endogenous Retrovirus env Gene in the Blood of Individuals with Schizophrenia by Using Nested RT-PCR

1.
1.State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
2.
Renmin Hospital, Wuhan University, Wuhan 430060, China

Abstract

The association between transcriptional activation of HERV and the develop- ment of schizophrenia was investigated. Nested RT-PCR was used to detect the preval- ence of HERV env RNA in the PBMCs of individuals with recent-onset of schizophrenia and normal persons from central and southern China. β-actin gene was used as an internal control. HERV env homologous sequences were found in the PBMCs of 8 of 28 (28.6%) individuals with recent-onset schizophrenia, while no env sequences were identified in any of the PBMCs obtained from 24 normal person (P0.001). The results suggested that transcriptional activation of HERV might be associated with the develop- pment of schizophr- enia in at least some patients.
- Human Endogenous Retroviruses (HERV)
- , Schizophrenia
- , Nested RT-PCR

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Detection of Human Endogenous Retrovirus env Gene in the Blood of Individuals with Schizophrenia by Using Nested RT-PCR

1. 1.State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
2. Renmin Hospital, Wuhan University, Wuhan 430060, China

Abstract: The association between transcriptional activation of HERV and the develop- ment of schizophrenia was investigated. Nested RT-PCR was used to detect the preval- ence of HERV env RNA in the PBMCs of individuals with recent-onset of schizophrenia and normal persons from central and southern China. β-actin gene was used as an internal control. HERV env homologous sequences were found in the PBMCs of 8 of 28 (28.6%) individuals with recent-onset schizophrenia, while no env sequences were identified in any of the PBMCs obtained from 24 normal person (P0.001). The results suggested that transcriptional activation of HERV might be associated with the develop- pment of schizophr- enia in at least some patients.