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Volume 22 Issue 1
February 2007

    Yan SUN, Li-jun LI, Jing LI and Zhi LI. Inhibition of Hepatitis B Virus Replication by Rheum palmatum L. Ethanol Extract in a Stable HBV-producing Cell Line[J]. Virologica Sinica, 2007, 22(1): 14-20.
    Citation: Yan SUN, Li-jun LI, Jing LI, Zhi LI. Inhibition of Hepatitis B Virus Replication by Rheum palmatum L. Ethanol Extract in a Stable HBV-producing Cell Line .VIROLOGICA SINICA, 2007, 22(1) : 14-20.
    shu

    Inhibition of Hepatitis B Virus Replication by Rheum palmatum L. Ethanol Extract in a Stable HBV-producing Cell Line

    • 1.

      College of Life Sciences,Shaanxi Normal University,Xi'an,Shannxi,710062

    • 2.

      Bioengineering Institute,Jimei University,Xiamen,Fujian,361021

    • 3.

      Department of Applied Biology and Chemical Technology,The Hong Kong Polytechnic University,Hung Hom,Hong Kong

    • 4.

      Department of Infection Disease,Xiangfan Central Hospital,Xiangfan,Hubei,441021

    • 5.

      The Institute of Molecular Biology,The University of Hong Kong

    • Corresponding author: Zhi LI, lizhi@snnu.edu.cn
    • Received Date: 31 May 2006
      Accepted Date: 06 July 2006
    • Hepatitis B virus(HBV) infection is a severe health problem in the world. However,there is still not a satisfactory therapeutic strategy for the HBV infection. To search for new anti-HBV agents with higher efficacy and less side-effects,the inhibitory activities of traditional Chinese medicine Rheum palmatum L. ethanol extract(RPE) against HBV replication were investigated in this study. Quantitative real-time polymerase chain reaction(PCR) was employed to analyze the inhibitory activity of RPE against HBV-DNA replication in a stable HBV-producing cell line HepAD38; the expression levels of HBV surface antigen(HBsAg) and e antigen(HBeAg) were also determined by enzyme linked immunosorbent assay(ELISA) after RPE treatment. RPE could dose-dependently inhibit the production of HBV-DNA and HBsAg. The concentration of 50% inhibition(IC50) was calculated at 209.63,252.53μg /mL,respectively. However,its inhibitory activity against HBeAg expression was slight even at high concentrations. RPE had a weak cytotoxic effect on HepAD38 cells(CC50= 1 640μg /mL) and the selectivity index(SI) was calculated at 7.82. Compared with two anthraquinone derivatives emodin and rhein,RPE showed higher ability of anti-HBV and weaker cytotoxicity. So Rheum palmatum L. might possess other functional agents which could effectively inhibit HBV-DNA replication and HBsAg expression. Further purification of the active agents,identification and modification of their structures to improve the efficacy and decrease the cytotoxicity are required.
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      Inhibition of Hepatitis B Virus Replication by Rheum palmatum L. Ethanol Extract in a Stable HBV-producing Cell Line

        Corresponding author: Zhi LI, lizhi@snnu.edu.cn
      • 1. College of Life Sciences,Shaanxi Normal University,Xi'an,Shannxi,710062
      • 2. Bioengineering Institute,Jimei University,Xiamen,Fujian,361021
      • 3. Department of Applied Biology and Chemical Technology,The Hong Kong Polytechnic University,Hung Hom,Hong Kong
      • 4. Department of Infection Disease,Xiangfan Central Hospital,Xiangfan,Hubei,441021
      • 5. The Institute of Molecular Biology,The University of Hong Kong
      • Received Date: 31 May 2006
      • Accepted Date: 06 July 2006

      Abstract: Hepatitis B virus(HBV) infection is a severe health problem in the world. However,there is still not a satisfactory therapeutic strategy for the HBV infection. To search for new anti-HBV agents with higher efficacy and less side-effects,the inhibitory activities of traditional Chinese medicine Rheum palmatum L. ethanol extract(RPE) against HBV replication were investigated in this study. Quantitative real-time polymerase chain reaction(PCR) was employed to analyze the inhibitory activity of RPE against HBV-DNA replication in a stable HBV-producing cell line HepAD38; the expression levels of HBV surface antigen(HBsAg) and e antigen(HBeAg) were also determined by enzyme linked immunosorbent assay(ELISA) after RPE treatment. RPE could dose-dependently inhibit the production of HBV-DNA and HBsAg. The concentration of 50% inhibition(IC50) was calculated at 209.63,252.53μg /mL,respectively. However,its inhibitory activity against HBeAg expression was slight even at high concentrations. RPE had a weak cytotoxic effect on HepAD38 cells(CC50= 1 640μg /mL) and the selectivity index(SI) was calculated at 7.82. Compared with two anthraquinone derivatives emodin and rhein,RPE showed higher ability of anti-HBV and weaker cytotoxicity. So Rheum palmatum L. might possess other functional agents which could effectively inhibit HBV-DNA replication and HBsAg expression. Further purification of the active agents,identification and modification of their structures to improve the efficacy and decrease the cytotoxicity are required.

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