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Volume 22 Issue 1
February 2007

    Yi-mei CAO, Zeng-jun LU, Zai-xin LIU and Qing-ge XIE. Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals[J]. Virologica Sinica, 2007, 22(1): 74-79.
    Citation: Yi-mei CAO, Zeng-jun LU, Zai-xin LIU, Qing-ge XIE. Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals .VIROLOGICA SINICA, 2007, 22(1) : 74-79.
    shu

    鉴别口蹄疫病毒感染与免疫动物的三种不同 ELISA试剂盒的比较研究

    • 中国农业科学院兰州兽医研究所农业部病毒学重点实验室,甘肃 兰州 730046

    • 通讯作者： 刘在新*, liukey@public.lz.gs.cn; 
    • 收稿日期： 2006-04-14
      录用日期： 2006-05-22
    • 摘要：为了进一步验证本实验室研制的鉴别口蹄疫病毒感染牛与免疫牛的FMD 3ABC-I-ELISA试剂盒的有效性，我们进行了该项研究。分别用ceditest?、 UBI?与本实验室研制的FMD 3ABC-I-ELISA三种口蹄疫非结构蛋白抗体检测ELISA试剂盒对不同的血清样品（非免疫健康牛血清、灭活疫苗免疫牛血清、人工感染牛血清）进行检测。初步确定其敏感性与特异性，并将结果进行比较。结果表明，FMD 3ABC-I-ELISA与ceditest?非结构蛋白抗体检测试剂盒的符合率在98.05％以上，而与UBI?的符合率为94.4％。对感染牛来说，FMD 3ABC-I-ELISA和ceditest?两种试剂盒的敏感性都达到100%，而UBI?敏感性较低，只有81.8%。对免疫牛和健康牛来说，三种试剂盒的特异性都在90% 以上。

    Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals

    • State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China

    • Corresponding author: Zai-xin LIU, liukey@public.lz.gs.cn
    • Received Date: 14 April 2006
      Accepted Date: 22 May 2006

      Fund Project: National "973" research project G199901190International Atomic Energy Agency (IAEA) 10697/R2National "973" research project 2005CB23201

    • A study was performed in order to validate FMDV 3ABC-I-ELISA kit developed in China for the differentiation of FMDV-infected animals from those had merely been vaccinated. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits, Ceditest? FMDV-NS (Ceditest? kit), UBI? FMDV NONSTRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI? kit) and FMDV 3ABC-I-ELISA kit developed at the LanZhou Veterinary Research Institute. The test parameters (sensitivity and specificity) of the three kits were primarily determined, and the result obtained from domestic FMD 3ABC-I-ELISA kit was compared with that of obtained from two foreign kits respectively. The results indicate that the coincidence rate between FMD 3ABC-I-ELISA and Ceditest? kit is 98.05%, and the coincidence rate between FMD 3ABC-I-ELISA and UBI? kit is 94.4%; the sensitivity of both Ceditest? and FMD 3ABC-I-ELISA kit is 100%. However, the sensitivity of UBI? kit is only 81.8%. With sera from naive or vaccinated, but non-infected animals, the specificity of all tests exceeds 90%.
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      3. Bergmann I E,Malirat V,Neitzert E,et al. 2000. Improvement of a serodiagnostic strategy for foot-and-mouth disease virus surveillance in cattle under systematic vaccination: a combined system of an indirect ELISA-3ABC with an enzyme-linked immunoelec-trotransfer blot assay[J]. Arch Virol,145(3): 473-489.
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      4. Clavijo A,Wright P,Kitching P. 2004. Developments in diag-nostic techniques for differen-tiating infection from Vaccination in foot-and-mouth disease [J]. The Vet J,167:9-22.
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      5. De Diego M,Brocchi E,Mackay D,et al. 1997. The non-structural polyprotein 3ABC of foot-and-mouth disease virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle[J]. Arch Virol,142(10): 2021-2033.
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      8. Mackay D K,Forsyth M A,Davies P R,et al. 1998. Differentiating infection from vaccination in foot-and-mouth disease using a panel of recombinant,non-structural proteins in ELISA[J]. Vaccine,16(5): 446-459.
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      9. Meyer R F,Babcock G D,Newman J F,et al. 1997. Baculovirus expressed 2C of foot-and-mouth disease virus has the potential for differentiating convalescent from vaccinated animals[J]. J Virol Methods,65(1): 33-43.
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      10. Mezencio J M,Babcock G D,Meyer R F,et al. 1998. Differentiating foot-and-mouth disease virus-infected from vaccinated animals with baculovirus-expressed specific proteins[J]. Vet Q,20(Suppl 2) S11-13.

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      12. Shen F,Chen P D,Walfield A M,et al. 1999. Differentiation of convalescent animals from those vaccinated against foot-and-mouth disease by a peptide ELISA[J].Vaccine,17(23-24): 3039-3049.
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      Comparison of Three ELISA Kits for the Differentiation of Foot-and-mouth Disease Virus-infected from Vaccinated Animals

        Corresponding author: Zai-xin LIU, liukey@public.lz.gs.cn
      • State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Virology of Ministry of Agriculture,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China
      • Received Date: 14 April 2006
      • Accepted Date: 22 May 2006
      Fund Project:  National "973" research project G199901190International Atomic Energy Agency (IAEA) 10697/R2National "973" research project 2005CB23201

      Abstract: A study was performed in order to validate FMDV 3ABC-I-ELISA kit developed in China for the differentiation of FMDV-infected animals from those had merely been vaccinated. Sets of sera from naive and vaccinated cattle as well as from cattle that had been infected were tested for antibodies against nonstructural proteins (NSPs) of FMDV by commercial diagnosis kits, Ceditest? FMDV-NS (Ceditest? kit), UBI? FMDV NONSTRUCTURAL PROTEIN ELISA DIRECTION INSERT (UBI? kit) and FMDV 3ABC-I-ELISA kit developed at the LanZhou Veterinary Research Institute. The test parameters (sensitivity and specificity) of the three kits were primarily determined, and the result obtained from domestic FMD 3ABC-I-ELISA kit was compared with that of obtained from two foreign kits respectively. The results indicate that the coincidence rate between FMD 3ABC-I-ELISA and Ceditest? kit is 98.05%, and the coincidence rate between FMD 3ABC-I-ELISA and UBI? kit is 94.4%; the sensitivity of both Ceditest? and FMD 3ABC-I-ELISA kit is 100%. However, the sensitivity of UBI? kit is only 81.8%. With sera from naive or vaccinated, but non-infected animals, the specificity of all tests exceeds 90%.

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