Citation: Shuaiyong Wang, Manzhu Wang, Lingxue Yu, Juan Wang, Jiecong Yan, Xinli Rong, Yanjun Zhou, Tongling Shan, Wu Tong, Guoxin Li, Hao Zheng, Guangzhi Tong, Hai Yu. Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus .VIROLOGICA SINICA, 2022, 37(4) : 627-630.  http://dx.doi.org/10.1016/j.virs.2022.04.009

Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus

  • Corresponding author: Hai Yu, haiyu_008@126.com
  • Received Date: 12 October 2021
    Accepted Date: 11 April 2022
    Available online: 02 May 2022
  • Highlights
    1. Identification of a reassortant EA H1N1 SIV (SD/18) which isolated from a pig farm in Shandong, north China.
    2. Phylogenetic analysis showed that SD/18 virus containing a complete internal gene cassette from pdm/09 virus.
    3. The results of pathogenicity in mice showed that the mortality rate of SD/18 virus in mice could reach 100%.
    4. The potential risk of EA lineage SIVs to humans is very high and we need to pay enough attention to the different reassortant EA H1N1 viruses.

  • 加载中
  • 10.1016j.virs.2022.04.009-ESM.docx
    1. Brown, I.H., 2000. The epidemiology and evolution of influenza viruses in pigs. Vet. Microbiol. 74, 29–46.

    2. Kothalawala, H., Toussaint, M.J., Gruys, E., 2006. An overview of swine influenza. Vet. Q. 28, 46–53.

    3. Kyriakis, C.S., Zhang, M., Wolf, S., Jones, L.P., Shim, B.S., Chocallo, A.H., Hanson, J.M., Jia, M., Liu, D., Tripp, R.A., 2017. Molecular epidemiology of swine influenza A viruses in the Southeastern United States, highlights regional differences in circulating strains. Vet. Microbiol. 211, 174–179.

    4. Killian, M.L., 2014. Hemagglutination assay for influenza virus. Methods Mol. Biol. 1161, 3–9.

    5. Li, X., Guo, L., Liu, C., Cheng, Y., Kong, M., Yang, L., Zhuang, Z., Liu, J., Zou, M., Dong, X., Su, X., Gu, Q., 2019. Human infection with a novel reassortant Eurasian-avian lineage swine H1N1 virus in northern China. Emerg. Microb. Infect. 8, 1535–1545.

    6. Liang, H., Lam, T.T., Fan, X., Chen, X., Zeng, Y., Zhou, J., Duan, L., Tse, M., Chan, C.H., Li, L., Leung, T.Y., Yip, C.H., Cheung, C.L., Zhou, B., Smith, D.K., Poon, L.L., Peiris, M., Guan, Y., Zhu, H., 2014. Expansion of genotypic diversity and establishment of 2009 H1N1 pandemic-origin internal genes in pigs in China. J. Virol. 88, 10864–10874.

    7. Parys, A., Vandoorn, E., King, J., Graaf, A., Pohlmann, A., Beer, M., Harder, T., Van Reeth, K., 2021. Human infection with Eurasian avian-like swine influenza A(H1N1) virus, The Netherlands, september 2019. Emerg. Infect. Dis. 27, 939–943.

    8. Pensaert, M., Ottis, K., Vandeputte, J., Kaplan, M.M., Bachmann, P.A., 1981. Evidence for the natural transmission of influenza A virus from wild ducts to swine and its potential importance for man. Bull. World Health Organ. 59, 75–78.

    9. Peacock, T.P., Swann, O.C., Salvesen, H.A., Staller, E., Leung, P.B., Goldhill, D.H., Zhou, H., Lillico, S.G., Whitelaw, C.B.A., Long, J.S., Barclay, W.S., 2020. Swine ANP32A supports avian influenza virus polymerase. J. Virol. 94.

    10. Shope, R.E., 1931. Swine influenza: I. Experimental transmission and pathology. J. Exp. Med. 54, 349–359.

    11. Smith, G.J., Vijaykrishna, D., Bahl, J., Lycett, S.J., Worobey, M., Pybus, O.G., Ma, S.K., Cheung, C.L., Raghwani, J., Bhatt, S., Peiris, J.S., Guan, Y., Rambaut, A., 2009. Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature 459, 1122–1125.

    12. Sun, H., Xiao, Y., Liu, J., Wang, D., Li, F., Wang, C., Li, C., Zhu, J., Song, J., Sun, H., Jiang, Z., Liu, L., Zhang, X., Wei, K., Hou, D., Pu, J., Sun, Y., Tong, Q., Bi, Y., Chang, K.C., Liu, S., Gao, G.F., Liu, J., 2020. Prevalent Eurasian avian-like H1N1 swine influenza virus with 2009 pandemic viral genes facilitating human infection. Proc. Natl. Acad. Sci. U. S. A. 117, 17204–17210.

    13. Sun, Y.F., Wang, X.H., Li, X.L., Zhang, L., Li, H.H., Lu, C., Yang, C.L., Feng, J., Han, W., Ren, W.K., Tian, X.X., Tong, G.Z., Wen, F., Li, Z.J., Gong, X.Q., Liu, X.M., Ruan, B.Y., Yan, M.H., Yu, H., 2016. Novel triple-reassortant H1N1 swine influenza viruses in pigs in Tianjin, Northern China. Vet. Microbiol. 183, 85–91.

    14. Vijaykrishna, D., Poon, L.L., Zhu, H.C., Ma, S.K., Li, O.T., Cheung, C.L., Smith, G.J., Peiris, J.S., Guan, Y., 2010. Reassortment of pandemic H1N1/2009 influenza A virus in swine. Science 328, 1529.

    15. Vijaykrishna, D., Smith, G.J., Pybus, O.G., Zhu, H., Bhatt, S., Poon, L.L., Riley, S., Bahl, J., Ma, S.K., Cheung, C.L., Perera, R.A., Chen, H., Shortridge, K.F., Webby, R.J., Webster, R.G., Guan, Y., Peiris, J.S., 2011. Long-term evolution and transmission dynamics of swine influenza A virus. Nature 473, 519–522.

    16. Vincent, A., Awada, L., Brown, I., Chen, H., Claes, F., Dauphin, G., Donis, R., Culhane, M., Hamilton, K., Lewis, N., Mumford, E., Nguyen, T., Parchariyanon, S., Pasick, J., Pavade, G., Pereda, A., Peiris, M., Saito, T., Swenson, S., Van Reeth, K., Webby, R., Wong, F., Ciacci-Zanella, J., 2014. Review of influenza A virus in swine worldwide: a call for increased surveillance and research. Zoonoses Public Health 61, 4–17.

    17. Wang, D.Y., Qi, S.X., Li, X.Y., Guo, J.F., Tan, M.J., Han, G.Y., Liu, Y.F., Lan, Y., Yang, L., Huang, W.J., Cheng, Y.H., Zhao, X., Bai, T., Wang, Z., Wei, H.J., Xiao, N., Shu, Y.L., 2013. Human infection with Eurasian avian-like influenza A(H1N1) virus, China. Emerg. Infect. Dis. 19, 1709–1711.

    18. Zhu, W., Zhang, H., Xiang, X., Zhong, L., Yang, L., Guo, J., Xie, Y., Li, F., Deng, Z., Feng, H., Huang, Y., Hu, S., Xu, X., Zou, X., Li, X., Bai, T., Chen, Y., Li, Z., Li, J., Shu, Y., 2016. Reassortant Eurasian avian-like influenza A(H1N1) virus from a severely ill child, hunan province, China, 2015. Emerg. Infect. Dis. 22, 1930–1936.

  • 加载中

Article Metrics

Article views(2121) PDF downloads(20) Cited by()

Related
Proportional views

    Genetic characterization and pathogenicity of a reassortant Eurasian avian-like H1N1 swine influenza virus containing an internal gene cassette from 2009 pandemic H1N1 virus

      Corresponding author: Hai Yu, haiyu_008@126.com
    • a Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 518, Ziyue Road, Minhang District, Shanghai, 200241, China;
    • b Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China

    Abstract: Highlights
    1. Identification of a reassortant EA H1N1 SIV (SD/18) which isolated from a pig farm in Shandong, north China.
    2. Phylogenetic analysis showed that SD/18 virus containing a complete internal gene cassette from pdm/09 virus.
    3. The results of pathogenicity in mice showed that the mortality rate of SD/18 virus in mice could reach 100%.
    4. The potential risk of EA lineage SIVs to humans is very high and we need to pay enough attention to the different reassortant EA H1N1 viruses.

    Reference (18) Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return