Growth Characteristics and High Titer Culture of Grass Carp Hemorrhage Virus (GCHV)-873 in Vitro
Abstract: Some biological properties of Grass Carp Hemorrhage Virus (GCHV)-873have been studied in this report. This virus could make grass carp kidney cells(CIK) to produce a unique cytopathic effect. At 28℃ its logarithmic growthphase occured 12 to 48 hr after infection. The virus titer reached 1.78×10~(11)PFU/mlwhen the MOI was approximately 0.05PFU/cell. There were slight differencesin virus titer under incubation of 25℃, 28℃and 30℃ respectively, and thesimilar results in 5% CO_2 or in normal atmosphere.