Citation: DUAN Yan-Ping, CHEN Xiu-Zhu, Wu Yuan-Ming. Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody .VIROLOGICA SINICA, 1990, 5(1) : 36.

Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody

  • Available online: 05 March 1990
  • Two indirect immunofluorescent assays(IIFA), using a monoclonal antibody directed against protein antigen (McAb-20k)and specific positive sera of human cytomegalovirus(HCMV)respectively, were applied for the rapid detection of the virus from fifty urine specimens(28 cases)inoculated onto human embryonic fibroblast. By the former procedure g of 28 cases were positive at 48 hours postinfection and all of them developed cytopathic effect; by the latter 11 were positive at 6 days postinfection and only 10 cases...

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    Detection of the Antigen of Human Cytomegalovirus Isolates in Cell Culture by Indirect Immunofluorescent Assay Using Monoclonai Antibody

    • 1. Department of Microbiology
    • 2. Tongji Medical University

    Abstract: Two indirect immunofluorescent assays(IIFA), using a monoclonal antibody directed against protein antigen (McAb-20k)and specific positive sera of human cytomegalovirus(HCMV)respectively, were applied for the rapid detection of the virus from fifty urine specimens(28 cases)inoculated onto human embryonic fibroblast. By the former procedure g of 28 cases were positive at 48 hours postinfection and all of them developed cytopathic effect; by the latter 11 were positive at 6 days postinfection and only 10 cases...

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