Study on the Purification Techniques for Strawberry Pseudo-Mild Yellow Edge Virus

The isolated strawberry pseudo-mild yellow edge virus(SPMYEV)was maintained in the strawberry-Fragaria vesca L.var. UC-4, and was successfully extracted and purified through homogenization by polyethyleneglycol (PEG) precipitation, differential centrifugation in the sucrose cushion and continuous suerose density gradient centrifugation. The purified preparations cotained flexous rod-shaped virus particles mainly 650nm in length and 12—13nm in width. The virus showed a typitical UV spectrum of nueleoprotein ...