Citation: YANG Zhan-Qiu, XIAO Hong, WEN Li. Establishment of method for detection of respiratory syncytical virus RNA using reverse- transcription polymerase chain reaction .VIROLOGICA SINICA, 1997, 12(3) : 213.

Establishment of method for detection of respiratory syncytical virus RNA using reverse- transcription polymerase chain reaction

  • Available online: 05 September 1997
  • A method of reverse - transcription polymerase chain reaction(RT - PCR) was established for detection of respiratory syncytical virus (RSV) RNA genome, The primers were designed based on the nucleotide sequence of RSV N gene. The results showed that the primers can't amplify the genomes of influenza virus and parainfluenza virus, but it can detect RSV genome in naSopharyngeal aspirate samples in infants and young children. This method is better than virus isolation. Its specificity is similar to immunofluorescence assay. The results indicated that the RTPCR for RSV RNA is specific, rapid and sensitive. It is suitable for clinical diagnosis of RSV infection.

  • 加载中
  • 加载中

Article Metrics

Article views(4114) PDF downloads(936) Cited by()

Related
Proportional views

    Establishment of method for detection of respiratory syncytical virus RNA using reverse- transcription polymerase chain reaction

    • 1. Hubei Medical Virology University

    Abstract: A method of reverse - transcription polymerase chain reaction(RT - PCR) was established for detection of respiratory syncytical virus (RSV) RNA genome, The primers were designed based on the nucleotide sequence of RSV N gene. The results showed that the primers can't amplify the genomes of influenza virus and parainfluenza virus, but it can detect RSV genome in naSopharyngeal aspirate samples in infants and young children. This method is better than virus isolation. Its specificity is similar to immunofluorescence assay. The results indicated that the RTPCR for RSV RNA is specific, rapid and sensitive. It is suitable for clinical diagnosis of RSV infection.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return