Citation: HUANG Chang-Xing, YANG Wei-Song, HANG Chang-Shou, BAI Xue-Fan, LI Guang-Yu. Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector .VIROLOGICA SINICA, 1997, 12(4) : 322-328.

Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector

  • Available online: 05 December 1997
  • For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for constructingGl and G1 expression plasmids, pGEX-4T-l was a fusion-protein expression vector phsmid.The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different.The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2.

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    Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector

    • 1. Tangdu Haspital ,The Fourth Military Medical University ,Xi'an 710038,China

    Abstract: For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for constructingGl and G1 expression plasmids, pGEX-4T-l was a fusion-protein expression vector phsmid.The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different.The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2.

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