Expression of Hantaan Virus Envelope Glycoproteins G1 and G2 in E .coli with Differential Expression Vector
Abstract: For increasing the expression level of Hantaan vitus envelope glycoproteins GI and G2 in E.coli, the expression vector pKK223-3 and pGEX-4T -1 were used for constructingGl and G1 expression plasmids, pGEX-4T-l was a fusion-protein expression vector phsmid.The results showed that the expression levels of hantaan virus envelope glycoproteins G1 a nd G2 w ith differential expression vector plasmids were riot ohvions different.The exprssion levels of the portion of GI andG2 proteinswere about the same with the complete G1 and G2.