Morphology and Morphogenesis of Manderin fish Virus

The structure and morphogensis of Mandarin fish( Siniperca chuats ) virus were studied.The matured virons were about 135nm±10 in diameter with envelope outside.The intact virus is composed of three parts,its nucleocapsid was about 90nm±5,and the envelope was around 18nm±3,the air space between core and envelope was about 27nm±2?The morphogenesis of the virus was observed in the ultra thin section of spleen tissue cells with outbreak lethal disease,which were collected in different stages of infected mandarin fish.At early stage infected fish section,it showed typical virus entry into cell cytoplasm via viropexis.There were many viromatrix,unmatured,matured and viron released phases,which were found in middle and late phase of diseased fish.In addtion,the same virions were also detected in diseased kidney,liver,heart and gill tissue.Artificial infected fish also caused acute lethal disease with same virus found in its tissue section.