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Volume 19 Issue 4
August 2004
    Citation: . The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection .VIROLOGICA SINICA, 2004, 19(4) : 404-406.
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    The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection

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    • 1.

      1. Lanzhou Veterinary Research Institute, Lanzhou 730046, China

    • 2.

      North-West Sci-Tech University of Agriculture and Forestry, Yanling 710021, China

    • Available online: 25 August 2004
    • Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.
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      The Culture Characteristics of Classical swine fever virus C-strain(Derived from Spleen) in SK6 Cell and Its Detection

      • 1. 1. Lanzhou Veterinary Research Institute, Lanzhou 730046, China
      • 2. North-West Sci-Tech University of Agriculture and Forestry, Yanling 710021, China

      Abstract: Enzyme-linked immunosorbent assay(ELISA), direct florescent antibody staining, and RT-PCT were used to detect growth characteristics of Cassical swine fever virus C-strain (Derived from Spleen) in SK6 cell. The results indicated than C-strain (Derived from Spleen) can grow in SK6 cell at a lower level. Direct florescent antibody staining method was not suitable for the detection of attenuated lapinized C-strain. The study provided a primary guide for the detection of attenuated classical swine fever virus. It also supplies an elementary foundation for the study of its growth characteristic in SK6.

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