A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Pei Huang; Zanheng Huang; Meihui Liu; Yujie Bai; Hongli Jin; Jingbo Huang; Xingqi Liu; Zhenhong Guan; Ming Duan; Haili Zhang; Yuanyuan Li; Sandra Chiu; Hualei Wang

doi:10.1016/j.virs.2023.05.012

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Volume 38 Issue 4

August 2023

Citation: Pei Huang, Zanheng Huang, Meihui Liu, Yujie Bai, Hongli Jin, Jingbo Huang, Xingqi Liu, Zhenhong Guan, Ming Duan, Haili Zhang, Yuanyuan Li, Sandra Chiu, Hualei Wang. A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II .VIROLOGICA SINICA, 2023, 38(4) : 635-638. http://dx.doi.org/10.1016/j.virs.2023.05.012

A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Pei Huang ^a ,
Zanheng Huang ^a ,
Meihui Liu ^a ,
Yujie Bai ^a ,
Hongli Jin ^a ,
Jingbo Huang ^a ,
Xingqi Liu ^a ,
Zhenhong Guan ^a ,
Ming Duan ^a ,
Haili Zhang ^a ,
Yuanyuan Li ^a ,
Sandra Chiu ^{b
,,} ,
Hualei Wang ^{a
,,}

a. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China;
b. Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 244199, China

Corresponding author: Sandra Chiu, qiux@ustc.edu.cn
Hualei Wang, wanghualei@jlu.edu.cn
Received Date: 07 February 2023
Accepted Date: 31 May 2023
Available online: 05 June 2023

Abstract

Highlights
1. The newly developed visual assay panel is a rapid and reliable tool to differentiate the clade I and clade II MPXV within 25 min.
2. The panel combines recombinase-aid amplification and immunochromatography, and detects as low as 1 copy/μL recombinant plasmid.
3. Visual assay panel shows no cross-reactivity with orthopoxviruses and herpesvirus that infect humans, such as vaccinia virus.

Electronic Supplementary Material

10.1016j.virs.2023.05.012-ESM.docx
References
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8. Maksyutov, R.A., Gavrilova, E.V. Shchelkunov, S.N., 2016. Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay. J Virol Methods. 236, 215-220.
9. Mao, L., Ying, J., Selekon, B., Gonofio, E., Wang, X., Nakoune, E., Wong, G. Berthet, N., 2022. Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox Virus. Viruses. 14(10):2112.
10. Peng, Q., Xie, Y., Kuai, L., Wang, H., Qi, J., Gao, G.F. Shi, Y., 2023. Structure of monkeypox virus DNA polymerase holoenzyme. Science. 379, 100-105.
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A visual assay panel for the identification of monkeypox virus DNA belonging to the clades I and II

Corresponding author: Sandra Chiu, qiux@ustc.edu.cn

Corresponding author: Hualei Wang, wanghualei@jlu.edu.cn

a. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China;
b. Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 244199, China

Received Date: 07 February 2023
Accepted Date: 31 May 2023

Abstract: Highlights
1. The newly developed visual assay panel is a rapid and reliable tool to differentiate the clade I and clade II MPXV within 25 min.
2. The panel combines recombinase-aid amplification and immunochromatography, and detects as low as 1 copy/μL recombinant plasmid.
3. Visual assay panel shows no cross-reactivity with orthopoxviruses and herpesvirus that infect humans, such as vaccinia virus.