The Production of ldiotypic Antibody against EHFV and Immunoreaction of Anti-EHFV Induced by the Idotypic Antibody in Mouse

In this papar, the preparation of menoclonal antibodies using epidemic hemorrhagic fever virus J10 strain and anti-EHFV McAb antibody (idiotypic antibedy, Ab2)prcduced by rabbt immunized with 7 clones of McAb are described In vitro, Ab2 was able to combine specifically with serum of recovering patients with EHF and serum of rabbit immunized by EHFV. Ab2 separated and purified by means of affinity chromatography was used to immunize Balb/c mice. The immunized serum (Ab3) was determined by immunoflouresence a...