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Volume 24 Issue 5
October 2009

    Kelli Hoover and Michael J. Grove. Specificity of Developmental Resistance in Gypsy Moth (Lymantria dispar) to two DNA-Insect Viruses[J]. Virologica Sinica, 2009, 24(5): 493-500. doi: 10.1007/s12250-009-3053-0
    Citation: Kelli Hoover, Michael J. Grove. Specificity of Developmental Resistance in Gypsy Moth (Lymantria dispar) to two DNA-Insect Viruses .VIROLOGICA SINICA, 2009, 24(5) : 493-500.  http://dx.doi.org/10.1007/s12250-009-3053-0
    shu

    Specificity of Developmental Resistance in Gypsy Moth (Lymantria
    dispar) to two DNA-Insect Viruses*

    cstr: 32224.14.s12250-009-3053-0

    • The Pennsylvania State University, Department of Entomology, 501 ASI Building, University Park PA 16802, USA

    • 通讯作者： Kelli Hoover*, kxh25@psu.edu; 
    • 收稿日期： 2009-01-31
      录用日期： 2009-04-30

    • Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43% mortality occurred in newly molted fourth instars fed a dose of 5×106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18%-21%) with 5×106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80% mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.

    Specificity of Developmental Resistance in Gypsy Moth (Lymantria dispar) to two DNA-Insect Viruses

    • The Pennsylvania State University, Department of Entomology, 501 ASI Building, University Park PA 16802, USA

    • Corresponding author: Kelli Hoover, kxh25@psu.edu
    • Received Date: 31 January 2009
      Accepted Date: 30 April 2009

      Fund Project: the National Science Foundation USA IBN-0077710

    • Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43% mortality occurred in newly molted fourth instars fed a dose of 5×106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18%-21%) with 5×106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80% mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.
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      2. Burgerjon A, Biache G, Chaufaux J, et al. 1981. Sensibilité comparée, en fonction de leur ȃge, des chenilles de Lymantria dispar, Mamestra brassicae et Spodoptera littoralis aux virus de la polyhédrose nucleaire. Entomophaga, 26:47-58.
          doi: 10.1007/BF02371833

      3. Duan L, Otvos I S. 2001. Influence of larval age and virus concentration on mortality and sublethal effects of a nucleopolyhedrovirus on the western spruce budworm (Lepidoptera: Tortricidae). Environ Entomol, 30 (1): 136-146.
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      4. Engelhard E K, Volkman L E. 1995. Developmental resistance within fourth instar Trichoplusia ni orally inoculated with Autographa californica M nuclear polyhedrosis virus. Virology, 209: 384-389.
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      10. Grove M J, Hoover K. 2007. Intrastadial developmental resistance of third instar gypsy moths (Lymantria dispar L.) to L. dispar nucleopolyhedrovirus. Biol Contr. 40: 355-361.
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      11. Hoover K, Grove M, Su S. 2002. Systemic component to intrastadial developmental resistance to baculoviral disease in gypsy moth. Biol Contr, 25: 92-98.
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      12. King L A, Wilkinson N, Miller D P, et al. 1998. Entomopoxviruses. In: The Insect Viruses (Miller LK, Ball A, ed. ), New York, NY: Plenum Publishing. p1-29.

      13. Kirkpatrick B A, Washburn J O, Volkman L E. 1998. AcMNPV pathogenesis and developmental resistance in fifth instar Heliothis virescens. J Invert Pathol, 72: 63-72.
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      14. Lynn D E, Dougherty E M, McClintock J T, et al. 1988. Development of cell lines from various tissues of Lepidoptera. In: Invertebrate and Fish Tissue Culture (Mit-suhashi J, ed. ), Tokyo: Japan Scientific Societies Press. p239-242.

      15. McCarthy W J, Granados R R, Roberts D W. 1974. Isolation and characterization of entomopox virions from virus-containing inclusions of Amsacta moorei (Lepidoptera: Arctiidae). Virology, 59: 59-69.
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      Specificity of Developmental Resistance in Gypsy Moth (Lymantria dispar) to two DNA-Insect Viruses

        Corresponding author: Kelli Hoover, kxh25@psu.edu
      • The Pennsylvania State University, Department of Entomology, 501 ASI Building, University Park PA 16802, USA
      • Received Date: 31 January 2009
      • Accepted Date: 30 April 2009
      Fund Project:  the National Science Foundation USA IBN-0077710

      Abstract: Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43% mortality occurred in newly molted fourth instars fed a dose of 5×106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18%-21%) with 5×106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80% mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.

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