Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA

Zhe Ren; Shen Li; Qiao-li Wang; Yang-fei Xiang; Yun-xia Cui; Yi-fei Wang; Ren-bin Qi; Da-xiang Lu; Shu-min Zhang; Pei-zhuo Zhang

doi:10.1007/s12250-011-3162-9

February 2011

Zhe Ren, Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui, Yi-fei Wang, Ren-bin Qi, Da-xiang Lu, Shu-min Zhang and Pei-zhuo Zhang. Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA[J]. Virologica Sinica, 2011, 26(1): 40-46. doi: 10.1007/s12250-011-3162-9

Citation: Zhe Ren, Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui, Yi-fei Wang, Ren-bin Qi, Da-xiang Lu, Shu-min Zhang, Pei-zhuo Zhang. Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA .VIROLOGICA SINICA, 2011, 26(1) : 40-46. http://dx.doi.org/10.1007/s12250-011-3162-9

siRNA对HSV-1空斑形成及RR mRNA相对表达水平的影响

任哲 ^1,2,# ,
李深 ^1,3,# ,
王巧利 ^1,3 ,
向阳飞 ^1,3 ,
崔蕴霞 ^1,3 ,
王一飞 ^1,3,, ,
戚仁斌 ² ,
陆大祥 ^2,, ,
张庶民 ^4,, ,
张佩琢 ⁵

1.
暨南大学生物医药研究开发基地，广东广州，510632，中国;2.暨南大学医学院，广东广州，510632，中国;3基因工程药物国家工程研究中心，广东广州，510632，中国;4中国药品生物制品检定所，北京，100050，中国;5上海吉玛制药技术有限公司，上海，201203，中国

通讯作者： 王一飞, twang-yf@163.com; 陆大祥, ldx@jnu.edu.cn; 张庶民, zhangsmo@hotmail.com;
收稿日期： 2010-08-03
录用日期： 2010-09-30

摘要

RNA 干扰是一种由siRNA 介导的、转录后mRNA 水平关闭相应基因表达的序列特异性基因沉默机制。HSV-1病毒脱氧核糖核苷酸还原酶（RR）分别由UL39和UL40基因编码的大、小两个亚基组成，本研究应用化学合成的靶向UL39和UL40基因的siRNAs，高效、特异地沉默了RR mRNA的表达，抑制了HSV-1病毒的复制，为RNA干扰作为抑制HSV-1病毒复制的基因工具提供了一种新的可能。
- siRNA
- , HSV-1
- , RR
- , 基因

Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA

Zhe Ren ^1,2,# ,
Shen Li ^1,3,# ,
Qiao-li Wang ^1,3 ,
Yang-fei Xiang ^1,3 ,
Yun-xia Cui ^1,3 ,
Yi-fei Wang ^1,3,, ,
Ren-bin Qi ² ,
Da-xiang Lu ^2,, ,
Shu-min Zhang ^4,, ,
Pei-zhuo Zhang ⁵

1.
Biomedicine research and development center of Jinan University, Guangdong, Guangzhou, 510632, China
2.
Medical College of Jinan University, Guangdong, Guangzhou, 510632, China
3.
National Engineering Research Center of Genetic Medicine, Guangdong, Guangzhou, 510632, China
4.
National Institute for the Control of Pharmaceutical and Biological Products, Beijing, 100050, China
5.
Shanghai GenePharma Co., Ltd, Shanghai, 201203, China

Corresponding author: Yi-fei Wang, twang-yf@163.com Da-xiang Lu, ldx@jnu.edu.cn Shu-min Zhang, zhangsmo@hotmail.com
Received Date: 03 August 2010
Accepted Date: 30 September 2010

Fund Project: Chinese Academy of Sciences O807E21211The Nation “863” Program of China 2006AA02A226The Joint Funds of National Science Foundation of China U0632010Chinese Academy of Sciences O807B11211

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication
- Small interfering RNA (siRNA)
- , Herpes simplex virus type Ⅰ (HSV-1)
- , Ribonucleotide reductase (RR)
- , Gene

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