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Volume 26 Issue 3
June 2011

    Jun-jun Shao, Jing-feng Wang, Hui-yun Chang and Ji-xing Liu. Immune Potential of a Novel Multiple-epitope Vaccine to FMDV Type Asia 1 in Guinea Pigs and Sheep[J]. Virologica Sinica, 2011, 26(3): 190-197. doi: 10.1007/s12250-011-3174-0
    Citation: Jun-jun Shao, Jing-feng Wang, Hui-yun Chang, Ji-xing Liu. Immune Potential of a Novel Multiple-epitope Vaccine to FMDV Type Asia 1 in Guinea Pigs and Sheep .VIROLOGICA SINICA, 2011, 26(3) : 190-197.  http://dx.doi.org/10.1007/s12250-011-3174-0
    shu

    一种新型Asia 1口蹄疫病毒多表位疫苗对豚鼠的免疫效力研究

    cstr: 32224.14.s12250-011-3174-0
    • 1.

      甘肃农业大学动物医学院，兰州730070;2农业部畜禽病毒学重点开放实验室，家畜疫病病原生物学国家重点实验室，中国农业科学院兰州兽医研究，中国 兰州 730046

    • 为了研制一种安全高效适用于羊的Asia1型口蹄疫病毒重组亚单位疫苗，选用了口蹄疫病毒的VP1蛋白区的主要抗原表位（137-160和197-211）设计和人工合成了一个串联重复多表位基因，同时引入了具有重要生物学功能的生物大分子，羊免疫球蛋白重链恒定区作为蛋白载体，成功构建了两个原核pET-30a-RE和pET-30a-RE-oIgG，并获得了以包涵体形式表达的重组蛋白。以豚鼠为模型对该疫苗进行了免疫效力评价，结果显示，重要生物功能分子IgG能明显增强表位的免疫效力。重组蛋白RE-oIgG不仅能够诱导高水平的中和抗体和淋巴细胞发生增值反应，而且提供了完全保护。虽然重组蛋白RE不能提供保护，但能延迟疾病出现临川针状的时间，减轻疾病的严重性。因此，我们认为该疫苗研究策略将对未来设计口蹄疫病毒新型疫苗提供新的思路。

    Immune Potential of a Novel Multiple-epitope Vaccine to FMDV Type Asia 1 in Guinea Pigs and Sheep

    • 1.

      College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

    • 2.

      State key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • Corresponding author: Hui-yun Chang, changhuiyun@126.com Ji-xing Liu, liujixing@hotmail.com
    • Received Date: 05 November 2011
      Accepted Date: 31 March 2011

      Fund Project: Key Project of Science and Technology of Gansu Province 092NKDA030National High Technology Research and Development Program of China 2006AA10A204National Science and Technlogy Pillar Program 2006DAD06A03

    • To develop a safe and efficient recombinant subunit vaccine to foot-and-mouth disease virus (FMDV) type Asia 1 in sheep, a tandem repeated multiple-epitope gene consisting of residues 137-160 and 197-211 of the VP1 gene of FMDV was designed and artificially synthesized. The biologically functional molecule, the ovine IgG heavy constant region (oIgG) as a protein carrier was introduced for design of the multiple-epitope recombinant vaccine and recombinant expression plasmids pET-30a-RE and pET-30a-RE-oIgG were successfully constructed. The recombinant proteins, RE and RE-oIgG, were expressed as a formation of inclusion bodies in E. coli. The immune potential of this vaccine regime in guinea pigs and sheep was evaluated. The results showed that IgG could significantly enhance the immune potential of antigenic epitopes. The recombinant protein RE-oIgG could not only elicit the high levels of neutralizing antibodies and lymphocytes proliferation responses in the vaccinated guinea pigs, but confer complete protection in guinea pigs against virus challenge. Although the recombinant protein RE could not confer protection in the vaccinated animals, it could delay the appearance of the clinical signs and reduce the severity of disease. Inspiringly, the titers of anti-FMDV neutralizing antibodies elicited in sheep vaccinated with RE-oIgG was significantly higher than that for the RE vaccination. Therefore, we speculated that this vaccine formulation may be a promising strategy for designing a novel vaccine against FMDV in the future.
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      Immune Potential of a Novel Multiple-epitope Vaccine to FMDV Type Asia 1 in Guinea Pigs and Sheep

        Corresponding author: Hui-yun Chang, changhuiyun@126.com
        Corresponding author: Ji-xing Liu, liujixing@hotmail.com
      • 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
      • 2. State key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • Received Date: 05 November 2011
      • Accepted Date: 31 March 2011
      Fund Project:  Key Project of Science and Technology of Gansu Province 092NKDA030National High Technology Research and Development Program of China 2006AA10A204National Science and Technlogy Pillar Program 2006DAD06A03

      Abstract: To develop a safe and efficient recombinant subunit vaccine to foot-and-mouth disease virus (FMDV) type Asia 1 in sheep, a tandem repeated multiple-epitope gene consisting of residues 137-160 and 197-211 of the VP1 gene of FMDV was designed and artificially synthesized. The biologically functional molecule, the ovine IgG heavy constant region (oIgG) as a protein carrier was introduced for design of the multiple-epitope recombinant vaccine and recombinant expression plasmids pET-30a-RE and pET-30a-RE-oIgG were successfully constructed. The recombinant proteins, RE and RE-oIgG, were expressed as a formation of inclusion bodies in E. coli. The immune potential of this vaccine regime in guinea pigs and sheep was evaluated. The results showed that IgG could significantly enhance the immune potential of antigenic epitopes. The recombinant protein RE-oIgG could not only elicit the high levels of neutralizing antibodies and lymphocytes proliferation responses in the vaccinated guinea pigs, but confer complete protection in guinea pigs against virus challenge. Although the recombinant protein RE could not confer protection in the vaccinated animals, it could delay the appearance of the clinical signs and reduce the severity of disease. Inspiringly, the titers of anti-FMDV neutralizing antibodies elicited in sheep vaccinated with RE-oIgG was significantly higher than that for the RE vaccination. Therefore, we speculated that this vaccine formulation may be a promising strategy for designing a novel vaccine against FMDV in the future.

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