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Volume 30 Issue 6
December 2015

    Xiuling Ji, Chunjing Zhang, Anxiu Kuang, Jiankai Li, Yinshan Cui, Kunhao Qin, Lianbing Lin, Benxu Cheng, Qi Zhang and Yunlin Wei. Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China[J]. Virologica Sinica, 2015, 30(6): 457-459. doi: 10.1007/s12250-015-3674-4
    Citation: Xiuling Ji, Chunjing Zhang, Anxiu Kuang, Jiankai Li, Yinshan Cui, Kunhao Qin, Lianbing Lin, Benxu Cheng, Qi Zhang, Yunlin Wei. Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China .VIROLOGICA SINICA, 2015, 30(6) : 457-459.  http://dx.doi.org/10.1007/s12250-015-3674-4
    shu

    纳帕海湿地低温烈性噬菌体形态多样性研究

    • 1.

      .昆明理工大学生命科学与技术学院，昆明650500，中国

    • 2.

      Department of Biology, University of Texas Rio Grande Valley,

    • 3.

      Edinburg, Texas 78539, USA

    • 出版日期： 2015-12-16
    • 噬菌体分布广泛，具有重要的生态作用，但关于湿地生态系统中噬菌体形态多样性的研究非常有限。纳帕海是我国独有的低纬度、低温、高海拔季节性高原沼泽湿地，深处三江并流保护区腹地，同时又是我国长江上游地区的重要水源地，其地质和生物资源都十分丰富，是开展地质学和生物学研究的重要基地。本研究从纳帕海高原湿地分离获得18株低温噬菌体，其中6株属于肌尾噬菌体科，4株属于短尾噬菌体科，8株属于长尾噬菌体科。18株低温噬菌体对宿主均具有特异性，宿主菌分属于8个不同的属，其中尤以侵染假单胞菌属的噬菌体最多。18株低温噬菌体在头部直径、尾管直径、尾管长上均有差异。从噬菌体宿主菌的多样性及噬菌体电镜形态上均表明纳帕海湿地低温微生物资源十分丰富，多样性表现突出。为进一步开展噬菌体的低温适应性机制、噬菌体与宿主的相互作用及噬菌体与宿主的协同进化机制等研究提供了宝贵的实验材料。

    Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China

    • 1.

      Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

    • 2.

      Department of Biology, University of Texas Rio Grande Valley, Edinburg, Texas 78539, USA

    • 3.

      Regional Academic Heath Center, School of Medicine, University of Texas Rio Grande Valley, Edinburg, Texas 78541, USA

    • Corresponding author: Yunlin Wei, weiyunlin18@gmail.com
    • ORCID: 0000-0002-5473-5737
    • Published Date: 16 December 2015
    • In summary, rod-shaped bacterial cells and Pseudomonas spp. dominated the bacterial community in the Napahai plateau wetland. Based on our TEM results, bacteriophages with larger capsids (60–140 nm) and Siphoviridae members were dominant. We were able to isolate a batch of cultured cold-active bacteriophages and their host bacterial strains, which should provide opportunities for further qualitative or quantitative analysis of bacteriophage function in wetland ecology. Specifically, these new isolates can help clarify bacteriophage coldadaptation mechanisms, structure, and assembly, as well as improve our understanding of phage-host co-evolution and interactions.
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      1. Ackermann HW. 2007. Arch Virol, 152: 227-243.
          doi: 10.1007/s00705-006-0849-1

      2. Bettarel Y, Bouvy M, Dumont C, et al. 2006. App Environ Microbiol, 72: 5274-5282.
          doi: 10.1128/AEM.00863-06

      3. Brum JR, Steward GF. 2010. Microbial Ecol, 60: 636-643.
          doi: 10.1007/s00248-010-9688-4

      4. Danovaro R, Dell'Anno A, Corinaldesi C, et al. 2008. Nature, 454:1084-1087.
          doi: 10.1038/nature07268

      5. Demuth J, Neve H, Witzel KP. 1993. Appl Environ Microbiol, 59: 3378-3384.

      6. Ferla RL, Leonardi M. 2005. Mar Ecol Evol Persp, 26: 82-88.
          doi: 10.1111/mae.2005.26.issue-2

      7. Helton RR, Wang K, Kan JJ, et al. 2012. FEMS Microbiol Ecol, 79: 474-486.
          doi: 10.1111/fem.2011.79.issue-2

      8. Ji XL, Yu HY, Zhang Q, et al. 2015. Ann Microbiol, 65: 1789-1796.
          doi: 10.1007/s13213-014-1018-5

      9. Ram AS, Arnous B, Danger M, et al. 2010. FEMS Microbiol Ecol, 74: 83-92.
          doi: 10.1111/j.1574-6941.2010.00920.x

      10. Sawstrom C, Lisle J, Anesio AM, et al. 2008. Extremophiles, 12: 167-175.
          doi: 10.1007/s00792-007-0134-6

      11. Williamson KE, Radosevich M, Wommack KE. 2005. Appl Environ Microbiol, 71: 3119-3125.
          doi: 10.1128/AEM.71.6.3119-3125.2005

      12. Wommack KE, Colwell RR. 2000. Microbiol Mol Biol Rev, 64: 69-114.
          doi: 10.1128/MMBR.64.1.69-114.2000

      13. Xiang X, Chen L, Huang X, et al. 2005. J Virol, 79: 8677-8686.
          doi: 10.1128/JVI.79.14.8677-8686.2005

      14. Xu YQ, Liu Y, Pei JS, et al. 2015. Virol Sin, 30: 11-18.
          doi: 10.1007/s12250-014-3543-6

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      Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China

        Corresponding author: Yunlin Wei, weiyunlin18@gmail.com
      • 1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
      • 2. Department of Biology, University of Texas Rio Grande Valley, Edinburg, Texas 78539, USA
      • 3. Regional Academic Heath Center, School of Medicine, University of Texas Rio Grande Valley, Edinburg, Texas 78541, USA
      • Published Date: 16 December 2015

      Abstract: In summary, rod-shaped bacterial cells and Pseudomonas spp. dominated the bacterial community in the Napahai plateau wetland. Based on our TEM results, bacteriophages with larger capsids (60–140 nm) and Siphoviridae members were dominant. We were able to isolate a batch of cultured cold-active bacteriophages and their host bacterial strains, which should provide opportunities for further qualitative or quantitative analysis of bacteriophage function in wetland ecology. Specifically, these new isolates can help clarify bacteriophage coldadaptation mechanisms, structure, and assembly, as well as improve our understanding of phage-host co-evolution and interactions.

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