Lack of association between integrin α<sub>v</sub>β<sub>3</sub> gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China

Xiao-Ping Chen; Hai-Rong Xiong; Ni Zhu; Qing-Zhou Chen; Hui Wang; Chao-Jie Zhong; Mei-Rong Wang; Shuang Lu; Fan Luo; Wei Hou

doi:10.1007/s12250-016-3888-0

February 2017

Xiao-Ping Chen, Hai-Rong Xiong, Ni Zhu, Qing-Zhou Chen, Hui Wang, Chao-Jie Zhong, Mei-Rong Wang, Shuang Lu, Fan Luo and Wei Hou. Lack of association between integrin αvβ3 gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China[J]. Virologica Sinica, 2017, 32(1): 73-79. doi: 10.1007/s12250-016-3888-0

Citation: Xiao-Ping Chen, Hai-Rong Xiong, Ni Zhu, Qing-Zhou Chen, Hui Wang, Chao-Jie Zhong, Mei-Rong Wang, Shuang Lu, Fan Luo, Wei Hou. Lack of association between integrin α_vβ₃ gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China .VIROLOGICA SINICA, 2017, 32(1) : 73-79. http://dx.doi.org/10.1007/s12250-016-3888-0

湖北地区汉族人群中整合素α_vβ₃基因单核苷酸多态性与汉坦病毒感染肾综合征出血热的关联性研究

武汉大学基础医学院医学病毒学研究所/病毒学国家重点实验室

通讯作者： 侯炜, houwei@whu.edu.cn, ORCID: 0000-0003-1439-455X
收稿日期： 2016-10-09
录用日期： 2017-01-04
出版日期： 2016-10-18

摘要

肾综合征出血热（hemorrhagic fever with renal syndrome，HFRS）由汉坦病毒感染引起，我国是高发地区。β₃整合素为致病性汉坦病毒在易感细胞上的主要受体，由β₃亚基参与组成的整合素有α_Vβ₃和α_IIbβ₃两种。既往研究表明整合素α_IIbβ₃的单核苷酸多态性（SNP）与汉坦病毒感染有一定关联性，而整合素α_Vβ₃ SNP与汉坦病毒感染的关联性未见报道。本实验采用TaqMan SNP基因分型探针实时定量PCR和双向等位基因特异性PCR（BI-PASA）两种方法对整合素α_Vβ₃ SNP rs5918、rs3768777、rs3738919、rs13306487、rs5921进行基因分型，研究整合素α_Vβ₃ SNP 与HV感染的关联性。结果发现湖北地区汉族人群中HFRS患者整合素α_Vβ₃五个位点的单核苷酸基因多态性与汉坦病毒感染易感性及疾病严重程度无关联性。
- 肾综合征出血热
- , 单核苷酸基因多态性
- , 汉坦病毒
- , 整合素α_Vβ₃

Lack of association between integrin α_vβ₃ gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China

State Key Laboratory of Virology, Institute of Medical Virology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China

Corresponding author: Wei Hou, houwei@whu.edu.cn
ORCID: 0000-0003-1439-455X
Received Date: 09 October 2016
Accepted Date: 04 January 2017
Published Date: 18 October 2016

Abstract

Hantaviruses belong to the family Bunyaviridae and cause hemorrhagic fever with renal syndrome (HFRS) in humans. β3 integrins, including αVβ3 and αIIbβ3 integrins, act as receptors on endothelial cells and play key roles in cellular entry during the pathogenesis of hantaviruses. Previous study demonstrated that the polymorphisms of integrin αIIbβ3 are associated with susceptibility to hantavirus infection and the disease severity of HFRS in Shaanxi Province of China, rather than in Finland. However, the polymorphisms of integrin αvβ3 in patients with HFRS was incompletely understood. Here, we aimed to investigate the associations between polymorphisms in human integrin αvβ3 and HFRS in Han Chinese individuals. Ninety patients with HFRS and 101 healthy controls were enrolled in this study. Analysis of five single nucleotide polymorphism (SNP) sites (rs3768777 and rs3738919 on ITGAV; rs13306487, rs5921, and rs5918 on ITGB3) was performed by TaqMan SNP genotyping assays and bi-directional PCR allele-specific amplification method. No significant differences were observed between the HFRS group and controls regarding the genotype and allele frequency distributions of any of the five SNP sites, and no associations were found between ITGAV polymorphisms/genotypes and disease severity. In conclusion, our results implied that these five SNPs in the integrin αvβ3 gene were not associated with HFRS susceptibility or severity in Han Chinese individuals in Hubei Province.
- hemorrhagic fever with renal syndrome (HFRS)
- , polymorphisms
- , hantavirus
- , integrin α_vβ₃

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