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Volume 34 Issue 3
June 2019

    Jun Zhuang, Wenwu Lin, Christopher J. Coates, Pengxiang Shang, Taiyun Wei, Zujian Wu and Lianhui Xie. Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence[J]. Virologica Sinica, 2019, 34(3): 295-305. doi: 10.1007/s12250-019-00094-4
    Citation: Jun Zhuang, Wenwu Lin, Christopher J. Coates, Pengxiang Shang, Taiyun Wei, Zujian Wu, Lianhui Xie. Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence .VIROLOGICA SINICA, 2019, 34(3) : 295-305.  http://dx.doi.org/10.1007/s12250-019-00094-4
    shu

    香蕉束顶病毒运动蛋白B4被释放耦联寄主因子的功能性多肽的切割是毒力所需的

    • 1.

      福建农林大学闽台作物有害生物生态防控国家重点实验室

    • 2.

      福建农林大学植物病毒研究所

    • 3.

      英国威尔士旺西大学生物科学系

    • 通讯作者： 庄军, fafuzj@126.com, ORCID: 0000-0001-7915-7497
      ; 谢联辉, fjxlh@126.com, ORCID: 0000-0002-8840-7993
    • 收稿日期： 2018-10-15
      录用日期： 2019-01-18
      出版日期： 2019-03-13
    • 香蕉束顶病毒（BBTV）严重危害着世界香蕉生产。BBTV编码的蛋白B4是个致病因子。但是，其相关的分子机制尚未知。本研究中，我们发现功能性多肽可能通过蛋白酶切割从蛋白B4释放出来。位点特异性突变暗示着蛋白B4的功能性切割为B4介导植物矮化和败育的病状所必需。B4释放出来的C端片段能靶向偶联RuBP羧化酶大亚基（RbcL）和涉及蛋白合成的延伸因子2（EF2）。这样，从B4（也是一前体）游离出来的多肽可作为影响BBTV病原与植物寄主互作的非经典性修饰子。

    Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence

    • 1.

      State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China

    • 2.

      Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China

    • 3.

      Department of Biosciences, College of Science, Swansea University, Swansea SA2 8PP, Wales, UK

    • Corresponding author: Jun Zhuang, fafuzj@126.com Lianhui Xie, fjxlh@126.com
    • ORCID: 0000-0001-7915-7497; 0000-0002-8840-7993
    • Received Date: 15 October 2018
      Accepted Date: 18 January 2019
      Published Date: 13 March 2019
    • Banana bunchy top virus (BBTV) poses a serious danger to banana crops worldwide. BBTV-encoded protein B4 is a determinant of pathogenicity. However, the relevant molecular mechanisms underlying its effects remain unknown. In this study, we found that a functional peptide could be liberated from protein B4, likely via proteolytic processing. Site-directed mutagenesis indicated that the functional processing of protein B4 is required for its pathogenic effects, including dwarfism and sterility, in plants. The released protein fragment targets host proteins, such as the large subunit of RuBisCO (RbcL) and elongation factor 2 (EF2), involved in protein synthesis. Therefore, the peptide released from B4 (also a precursor) may act as a non-canonical modifier to influence host–pathogen interactions involving BBTV and plants.
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      Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence

        Corresponding author: Jun Zhuang, fafuzj@126.com
        Corresponding author: Lianhui Xie, fjxlh@126.com
      • 1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China
      • 2. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
      • 3. Department of Biosciences, College of Science, Swansea University, Swansea SA2 8PP, Wales, UK
      • Received Date: 15 October 2018
      • Accepted Date: 18 January 2019
      • Published Date: 13 March 2019

      Abstract: Banana bunchy top virus (BBTV) poses a serious danger to banana crops worldwide. BBTV-encoded protein B4 is a determinant of pathogenicity. However, the relevant molecular mechanisms underlying its effects remain unknown. In this study, we found that a functional peptide could be liberated from protein B4, likely via proteolytic processing. Site-directed mutagenesis indicated that the functional processing of protein B4 is required for its pathogenic effects, including dwarfism and sterility, in plants. The released protein fragment targets host proteins, such as the large subunit of RuBisCO (RbcL) and elongation factor 2 (EF2), involved in protein synthesis. Therefore, the peptide released from B4 (also a precursor) may act as a non-canonical modifier to influence host–pathogen interactions involving BBTV and plants.

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