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Volume 36 Issue 2
April 2021

    Xiaoli Wu, Mingyue Li, Yanfang Zhang, Boyun Liang, Junming Shi, Yaohui Fang, Zhengyuan Su, Mengmeng Li, Wenjing Zhang, Ling Xu, Jun Wang, Qiaoli Wu, Shuang Tang, Hualin Wang, Tao Zhang, Cheng Peng, Xin Zheng, Fei Deng and Shu Shen. Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes[J]. Virologica Sinica, 2021, 36(2): 300-310. doi: 10.1007/s12250-020-00289-0
    Citation: Xiaoli Wu, Mingyue Li, Yanfang Zhang, Boyun Liang, Junming Shi, Yaohui Fang, Zhengyuan Su, Mengmeng Li, Wenjing Zhang, Ling Xu, Jun Wang, Qiaoli Wu, Shuang Tang, Hualin Wang, Tao Zhang, Cheng Peng, Xin Zheng, Fei Deng, Shu Shen. Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes .VIROLOGICA SINICA, 2021, 36(2) : 300-310.  http://dx.doi.org/10.1007/s12250-020-00289-0
    shu

    发热板血小板减少综合征病毒SFTSV系统发育分析揭示了新的重组事件与迁移路线

    • 1.

      中国科学院武汉病毒研究所病毒学国家重点实验室和国家病毒资源中心

    • 2.

      中国科学院大学

    • 3.

      华中科技大学同济医学院附属协和医院感染与免疫研究所传染病科

    • 通讯作者： 郑昕, xinsunshine1011@aliyun.com, ORCID: http://orcid.org/0000-0003-1209-5281
      ; 邓菲, df@wh.iov.cn, ORCID: http://orcid.org/0000-0002-5385-083X
      ; 沈姝, shenshu@wh.iov.cn, ORCID: http://orcid.org/0000-0002-0013-5365
    • 收稿日期： 2020-05-08
      录用日期： 2020-07-13
      出版日期： 2020-09-22

    • 发热伴血小板减少综合症病毒（SFTSV）会引起人类严重的发热性疾病，最初在中国的中部和东部省份，以及后来在日本和韩国发现了该病毒。湖北省是中国中部主要的SFTS流行地区之一。这项研究报告了2017年从湖北省患者中分离出11株新的SFTSV毒株。基于包括该新毒株在内的SFTSV片段的完整编码序列，进行了广泛的系统发育分析。本研究表明，目前湖北省有五种不同基因型的SFTSV在流传，SFTSV毒株有15种重排模式，并且每种基因型的SFTSV相关的迁移途径较之前更为复杂。与之前报道湖北省SFTSV的进化事件独立于其他流行地区相比，本研究发现湖北省参与了更多的SFTSV的进化事件。同时本研究通过比较每种基因型内的SFTSV序列同一性和不同基因型间的SFTSV序列（以基因型C1作为代表性毒株）同一性，发现SFTSV毒株的进一步分化。随后分析SFSTV毒株蛋白氨基酸在基因型，毒株或簇上存在的差异，分析可能与SFTSV毒株/基因型的不同生物学活性有关。总之，我们分析了湖北省SFTSV系统发育进化的现状，并讨论了与SFTSV进化相关的可能事件。为深入了解SFTSV进化提供了基础，引起人们在未来研究中选择适当的SFTSV毒株的关注。

    Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes

    • 1.

      State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China

    • 2.

      University of Chinese Academy of Sciences, Beijing 100049, China

    • 3.

      Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology, Wuhan 430022, China

    • Corresponding author: Xin Zheng, xinsunshine1011@aliyun.com Fei Deng, df@wh.iov.cn Shu Shen, shenshu@wh.iov.cn
    • ORCID: http://orcid.org/0000-0003-1209-5281; http://orcid.org/0000-0002-5385-083X; http://orcid.org/0000-0002-0013-5365
    • Received Date: 08 May 2020
      Accepted Date: 13 July 2020
      Published Date: 22 September 2020

    • Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017. Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains. It was suggested that five different SFTSV genotypes were circulating in Hubei, and 15 reassortment patterns and migration pathways correlated with each genotype were identified, which was more than previously recognized. Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions. Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1. Subsequently, amino acid variations specific for genotype(s), strain(s), or cluster(s) were inspected, which may be related to differential biological activity of SFTSV strains/genotypes. In conclusion, we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution. It provided an in-depth insight into SFTSV evolution, raising concerns for the use of proper SFTSV strains in future studies.

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    • 加载中

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      • 1. 

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      Novel SFTSV Phylogeny Reveals New Reassortment Events and Migration Routes

        Corresponding author: Xin Zheng, xinsunshine1011@aliyun.com
        Corresponding author: Fei Deng, df@wh.iov.cn
        Corresponding author: Shu Shen, shenshu@wh.iov.cn
      • 1. State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
      • 2. University of Chinese Academy of Sciences, Beijing 100049, China
      • 3. Department of Infectious Diseases, Institute of Infection and Immunology, Union Hospital, Tongji Medical College, HuaZhong University of Science and Technology, Wuhan 430022, China
      • Received Date: 08 May 2020
      • Accepted Date: 13 July 2020
      • Published Date: 22 September 2020

      Abstract: 

      Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the isolation of 11 new SFTSV strains from patients in Hubei Province collected in 2017. Extensive phylogenetic analyses were conducted based on the complete coding sequences of SFTSV segments including the new strains. It was suggested that five different SFTSV genotypes were circulating in Hubei, and 15 reassortment patterns and migration pathways correlated with each genotype were identified, which was more than previously recognized. Hubei Province was more involved in the evolutionary events of SFTSV than that previously thought in which the evolutionary events of SFTSV were reported to be independent from those in other epidemic regions. Further divergence of SFTSV strains was suggested by pairwise comparison of SFTSV sequences from each genotype and sequence identity normalized to representative strain in genotype C1. Subsequently, amino acid variations specific for genotype(s), strain(s), or cluster(s) were inspected, which may be related to differential biological activity of SFTSV strains/genotypes. In conclusion, we analyzed the current status of SFTSV phylogeny in Hubei Province and discussed the possible events correlated to SFTSV evolution. It provided an in-depth insight into SFTSV evolution, raising concerns for the use of proper SFTSV strains in future studies.

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