Optimization and applications of an <em>in vivo</em> bioluminescence imaging model of influenza A virus infections

Xiaojing Lin; Murong Zhu; Xiujuan Zhao; Longlong Si; Meiyue Dong; Varada Anirudhan; Qinghua Cui; Lijun Rong; Ruikun Du

doi:10.1016/j.virs.2023.04.007

August 2023

. doi: 10.1016/j.virs.2023.04.007

Citation: Xiaojing Lin, Murong Zhu, Xiujuan Zhao, Longlong Si, Meiyue Dong, Varada Anirudhan, Qinghua Cui, Lijun Rong, Ruikun Du. Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections .VIROLOGICA SINICA, 2023, 38(4) : 631-634. http://dx.doi.org/10.1016/j.virs.2023.04.007

一种流感小鼠活体成像模型的优化和应用

林晓静 ^a ,
朱慕蓉 ^a ,
赵秀娟 ^a ,
司龙龙 ^b ,
董梅月 ^a ,
Varada Anirudhan ^c ,
崔清华 ^a,d,, ,
荣立军 ^c,, ,
杜瑞坤 ^a,d,,

a. 山东中医药大学中医药创新研究院;
b. 中国科学院深圳先进技术研究院;
c. 伊利诺伊大学芝加哥校区医学院;
d. 山东中医药大学青岛中医药科学院

通讯作者： 崔清华, cuiqinghua@sdutcm.edu.cn; 荣立军, lijun@uic.edu; 杜瑞坤, ruikun@sdutcm.edu.cn
收稿日期： 2023-03-03
录用日期： 2023-04-26

摘要

流感病毒分节段基因组对于外源基因插入修饰的容纳能力较低，导致流感报告病毒构建难度增大，限制了流感小鼠活体成像模型的建立和应用。前期，我们通过开发一种“平衡补偿”策略，成功构建了携带萤火虫荧光素酶基因（Fluc）的重组流感报告病毒，进而建立了稳健的流感小鼠活体成像模型。本研究通过对该模型进行评价和优化，将其应用于探究流感病毒学基础及应用相关的重要科学问题。
- 活体成像
- , 动物模型
- , 流感病毒

Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections

Xiaojing Lin ^a ,
Murong Zhu ^a ,
Xiujuan Zhao ^a ,
Longlong Si ^b ,
Meiyue Dong ^a ,
Varada Anirudhan ^c ,
Qinghua Cui ^a,d,, ,
Lijun Rong ^c,, ,
Ruikun Du ^a,d,,

a. Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 50355, China;
b. CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China;
c. Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA;
d. Qingdao Academy of Chinese Medical Sciences, Shandong University of Traditional Chinese Medicine, Qingdao, 266122, China

Corresponding author: Qinghua Cui, cuiqinghua@sdutcm.edu.cn Lijun Rong, lijun@uic.edu Ruikun Du, ruikun@sdutcm.edu.cn
Received Date: 03 March 2023
Accepted Date: 26 April 2023

Abstract

Highlights
1. The in vivo BLI model of IAV infections can simplify the determination of viral load in living animals;
2. The in vivo BLI model of IAV infections allow longitudinal measurements of virus infection/spread in living animals;
3. The in vivo BLI model of IAV infections improved the throughput of animal models;
4. The advanced BLI models can facilitate studies in both basic and applied virology.

References
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7. Govorkova, E.A., Leneva, I.A., Goloubeva, O.G., Bush, K., Webster, R.G., 2001. Comparison of efficacies of RWJ-270201, zanamivir, and oseltamivir against H5N1, H9N2, and other avian influenza viruses. Antimicrob Agents Chemother 45, 2723-2732.
8. Harris, R., Yang, J., Pagan, K., Cho, S.J., Stout-Delgado, H., 2021. Antiviral Gene Expression in Young and Aged Murine Lung during H1N1 and H3N2. Int J Mol Sci 22.
9. Iuliano, A.D., Roguski, K.M., Chang, H.H., Muscatello, D.J., Palekar, R., Tempia, S., Cohen, C., Gran, J.M., Schanzer, D., Cowling, B.J., Wu, P., Kyncl, J., Ang, L.W., Park, M., Redlberger-Fritz, M., Yu, H., Espenhain, L., Krishnan, A., Emukule, G., van Asten, L., Pereira da Silva, S., Aungkulanon, S., Buchholz, U., Widdowson, M.A., Bresee, J.S., 2018. Estimates of global seasonal influenza-associated respiratory mortality:a modelling study. Lancet 391, 1285-1300.
10. Kandasamy, M., Furlong, K., Perez, J.T., Manicassamy, S., Manicassamy, B., 2020. Suppression of Cytotoxic T Cell Functions and Decreased Levels of Tissue-Resident Memory T Cells during H5N1 Infection. J Virol 94.
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12. Mehle, A., 2015. Fiat Luc:Bioluminescence Imaging Reveals In Vivo Viral Replication Dynamics. PLoS Pathog 11, e1005081.
13. Si, L., Shen, Q., Li, J., Chen, L., Shen, J., Xiao, X., Bai, H., Feng, T., Ye, A.Y., Li, L., Zhang, C., Li, Z., Wang, P., Oh, C.Y., Nurani, A., Niu, S., Zhang, C., Wei, X., Yuan, W., Liao, H., Huang, X., Wang, N., Tian, W.X., Tian, H., Li, L., Liu, X., Plebani, R., 2022. Generation of a live attenuated influenza A vaccine by proteolysis targeting. Nat Biotechnol 40, 1370-1377.
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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections

Corresponding author: Qinghua Cui, cuiqinghua@sdutcm.edu.cn

Corresponding author: Lijun Rong, lijun@uic.edu

Corresponding author: Ruikun Du, ruikun@sdutcm.edu.cn

a. Innovative Institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 50355, China;

b. CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China;

c. Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA;

d. Qingdao Academy of Chinese Medical Sciences, Shandong University of Traditional Chinese Medicine, Qingdao, 266122, China

Received Date: 03 March 2023

Accepted Date: 26 April 2023

Abstract: Highlights
1. The in vivo BLI model of IAV infections can simplify the determination of viral load in living animals;
2. The in vivo BLI model of IAV infections allow longitudinal measurements of virus infection/spread in living animals;
3. The in vivo BLI model of IAV infections improved the throughput of animal models;
4. The advanced BLI models can facilitate studies in both basic and applied virology.

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一种流感小鼠活体成像模型的优化和应用

摘要

Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections

Abstract

References

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Electronic Supplementary Material

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Related

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通讯作者: 陈斌, bchen63@163.com

Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections

Corresponding author: Qinghua Cui, cuiqinghua@sdutcm.edu.cn

Corresponding author: Lijun Rong, lijun@uic.edu

Corresponding author: Ruikun Du, ruikun@sdutcm.edu.cn

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