First detection of cutavirus DNA in stools of patients with rheumatic diseases in Guangzhou, China

Yongzhi Li; Liting Zheng; Huan He; Husheng Xiong; Jiaqi Chen; Hengbiao Sun; Caiyun Chen; Qiushuang Li; Jiaqi Fu; Fei Wu; Yuhan Gao; Juxian Xian; Minyi Liang; Gang Xiao; Qing Chen

doi:10.1016/j.virs.2023.10.006

December 2023

. doi: 10.1016/j.virs.2023.10.006

Citation: Yongzhi Li, Liting Zheng, Huan He, Husheng Xiong, Jiaqi Chen, Hengbiao Sun, Caiyun Chen, Qiushuang Li, Jiaqi Fu, Fei Wu, Yuhan Gao, Juxian Xian, Minyi Liang, Gang Xiao, Qing Chen. First detection of cutavirus DNA in stools of patients with rheumatic diseases in Guangzhou, China .VIROLOGICA SINICA, 2023, 38(6) : 860-867. http://dx.doi.org/10.1016/j.virs.2023.10.006

首次在广州地区的风湿病患者粪便中检测到库塔病毒DNA

李泳枝 ^a ,
郑丽婷 ^a ,
何欢 ^a ,
熊胡晟 ^a ,
陈佳琪 ^a ,
孙恒彪 ^b ,
陈彩云 ^a ,
李秋爽 ^a ,
傅嘉琪 ^a ,
伍飞 ^a ,
高钰涵 ^a ,
冼举贤 ^a ,
梁敏仪 ^a ,
肖刚 ^b,, ,
陈清 ^a,,

a. 南方医科大学公共卫生学院流行病学教研室;
b. 南方医科大学第三附属医院检验科

通讯作者： 肖刚, xiaogang2993@yeah.net; 陈清, qch.2009@163.com
收稿日期： 2023-02-13
录用日期： 2023-10-08

摘要

CuV是一种新发的原细小病毒，可能与腹泻以及皮肤T细胞淋巴瘤有关。风湿病患者可能由于免疫抑制而对该病毒易感。在2019年6月至2022年6月期间，我们针对中国广州某医院的风湿病住院患者和健康体检人群进行了一项描述性研究。对上述两组受试者的粪便样本进行CuV DNA检测以及对从电子病历中获取到的受试者人口学信息和粪便实验室检查结果进行分析。一共有505名风湿病患者和244名健康体检人群被纳入了该项研究。在风湿病患者中，CuV DNA的阳性检出率为5.74%（95%可信区间：4.03%~8.12%）；体检组未检出阳性。根据疾病分类，男性和类风湿关节炎或强直性脊柱炎患者更容易感染CuV （P < 0.01）。对结果进行校正后，男性仍然是CuV检出的显著因素，且调整后的比值比（OR）值为4.4（95%可信区间：1.7-11.4，P=0.002）。系统进化分析显示了CuV聚类成三个不同的分支，本研究中的其中一条序列被分离为单独的分支。结果表明，CuV DNA不易在普通人群中被检出，但其可能与风湿病密切相关。男性可能是CuV感染的危险因素。VP2区域序列被发现聚类成三个不同的系统发育分支，其中一个分支可能是一个新的基因型。
- 库塔病毒
- , 风湿病
- , 流行
- , 危险因素
- , 遗传特征

First detection of cutavirus DNA in stools of patients with rheumatic diseases in Guangzhou, China

Yongzhi Li ^a ,
Liting Zheng ^a ,
Huan He ^a ,
Husheng Xiong ^a ,
Jiaqi Chen ^a ,
Hengbiao Sun ^b ,
Caiyun Chen ^a ,
Qiushuang Li ^a ,
Jiaqi Fu ^a ,
Fei Wu ^a ,
Yuhan Gao ^a ,
Juxian Xian ^a ,
Minyi Liang ^a ,
Gang Xiao ^b,, ,
Qing Chen ^a,,

a. Guangdong Provincial Key Laboratory of Tropical Disease Research, Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou, 510515, China;
b. Clinical Laboratory of Third Affiliated Hospital of Southern Medical University, Guangzhou, 510500, China

Corresponding author: Gang Xiao, xiaogang2993@yeah.net Qing Chen, qch.2009@163.com
Received Date: 13 February 2023
Accepted Date: 08 October 2023

Abstract

Cutavirus (CuV) is a novel protoparvovirus possibly associated with diarrhea and cutaneous T-cell lymphomas. Patients with rheumatic disease are immunosuppressed and may be more vulnerable to pathogenic viruses. A descriptive study was conducted among hospitalized patients with rheumatic diseases and individuals undergoing medical health check-ups between June 2019 and June 2022 in Guangzhou, China. Stool samples of subjects were tested for CuV DNA. Demographic and fecal examination data of patients were obtained from electronic medical records. A total of 505 patients with rheumatic diseases and 244 individuals who underwent medical health check-ups were included in the study. Of the patients with rheumatic disease, 5.74% [95% confidence interval (CI): 4.03%–8.12%] were positive for CuV DNA, while no individual in the medical health check-up group was positive, indicating a close correlation between CuV and rheumatic disease. Men and patients with rheumatoid arthritis or ankylosing spondylitis, according to the disease classification, were more susceptible to being infected with CuV (P < 0.01). After adjustments, being male remained the only significant factor, with an adjusted odd ratio (OR) of 4.4 (95% CI: 1.7–11.4, P = 0.002). Phylogenetic analysis of the CuV VP2 sequences showed three diverse clades, one of which was segregated to be a single branching independent of previously known sequences, which is possible a new genotype.
- Cutavirus (CuV)
- , Rheumatic diseases
- , Prevalence
- , Risk factor
- , Genetic characterization

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Electronic Supplementary Material

10.1016j.virs.2023.10.006-ESM.doc