Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China

Min Guo; Kai Zhao; Xingwen Peng; Xiangyang He; Jin Deng; Bo Wang; Xinglou Yang; Libiao Zhang

doi:10.1016/j.virs.2023.11.003

December 2023

. doi: 10.1016/j.virs.2023.11.003

Citation: Min Guo, Kai Zhao, Xingwen Peng, Xiangyang He, Jin Deng, Bo Wang, Xinglou Yang, Libiao Zhang. Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China .VIROLOGICA SINICA, 2023, 38(6) : 868-876. http://dx.doi.org/10.1016/j.virs.2023.11.003

中国南方褐扁颅蝠中发现穿山甲冠状病毒HKU4r-CoV

郭敏 ^a ,
赵锴 ^b ,
彭兴文 ^a ,
何向阳 ^a ,
邓瑾 ^a ,
王博 ^c ,
杨兴娄 ^b,d,, ,
张礼标 ^a,,

a. 广东省科学院动物研究所;
b. 中国科学院昆明动物研究所;
c. 美国弗吉尼亚理工大学;
d. 湖北江夏实验室

通讯作者： 杨兴娄, yangxinglou@mail.kiz.ac.cn; 张礼标, zhanglb@giz.gd.cn
收稿日期： 2023-04-10
录用日期： 2023-11-10

摘要

野生动物冠状病毒（CoV）外溢事件目前受到公众的广泛关注，这加剧了对携带有多种病毒的蝙蝠开展冠状病毒调查的必要性。本研究在2016年和2017年期间共采集了位于中国云南省和广东省的9个地点20种蝙蝠的729份肛拭子样品，分析了样品中α冠状病毒和β冠状病毒的分子特征和遗传多样性。通过RT-PCR检测，在分布于6个地点的9种蝙蝠中发现58种（8.0%）冠状病毒。进一步利用Illumina测序平台获得了其中来自褐扁颅蝠（Tylonycteris robustula）的2个冠状病毒TyRo-CoV-162275和TyRo-CoV-162269的全长基因组序列。对这2个基因组的序列分析表明，TyRo-CoV-162275与马来亚穿山甲（Manis javanica）的冠状病毒MjHKU4r-CoV一致性最高（93.9%）；而TyRo-CoV-162269与贵州的褐扁颅蝠的HKU33-CoV一致性最高（94.1%）。更重要的是，在TyRo-CoV-162275的S蛋白序列中发现了furin蛋白酶切割位点，揭示该病毒可能与MERS-CoV一样，以二肽基肽酶-4（hDPP4）为受体感染人体细胞。这是首次对含furin蛋白酶切割位点的蝙蝠冠状病毒HKU4r-CoV的报道。这些发现拓展了我们对冠状病毒地理和宿主分布的认知。
- 冠状病毒
- , 穿山甲冠状病毒HKU4r-CoV
- , 蝙蝠冠状病毒HKU4r-CoV
- , 褐扁颅蝠
- , 外溢风险

Pangolin HKU4-related coronaviruses found in greater bamboo bats from southern China

Min Guo ^a ,
Kai Zhao ^b ,
Xingwen Peng ^a ,
Xiangyang He ^a ,
Jin Deng ^a ,
Bo Wang ^c ,
Xinglou Yang ^b,d,, ,
Libiao Zhang ^a,,

a. Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou, 510260, China;
b. Yunnan Key Laboratory of Biodiversity Information, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650023, China;
c. Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24060, USA;
d. Hubei Jiangxia Lab, Wuhan, 430071, China

Corresponding author: Xinglou Yang, yangxinglou@mail.kiz.ac.cn Libiao Zhang, zhanglb@giz.gd.cn
Received Date: 10 April 2023
Accepted Date: 10 November 2023

Abstract

Coronavirus (CoV) spillover originating from game animals, particularly pangolins, is currently a significant concern. Meanwhile, vigilance is urgently needed for coronaviruses carried by bats, which are known as natural reservoirs of many coronaviruses. In this study, we collected 729 anal swabs of 20 different bat species from nine locations in Yunnan and Guangdong provinces, southern China, in 2016 and 2017, and described the molecular characteristics and genetic diversity of alphacoronaviruses (αCoVs) and betacoronaviruses (βCoVs) found in these bats. Using RT-PCR, we identified 58 (8.0%) bat CoVs in nine bat species from six locations. Furthermore, using the Illumina platform, we obtained two representative full-length genomes of the bat CoVs, namely TyRo-CoV-162275 and TyRo-CoV-162269. Sequence analysis showed that TyRo-CoV-162275 shared the highest identity with Malayan pangolin (Manis javanica) HKU4-related coronaviruses (MjHKU4r-CoVs) from Guangxi Province, whereas TyRo-CoV-162269 was closely related to HKU33-CoV discovered in a greater bamboo bat (Tylonycteris robustula) from Guizhou Province. Notably, TyRo-CoV-162275 has a putative furin protease cleavage site in its S protein and is likely to utilize human dipeptidyl peptidase-4 (hDPP4) as a cell-entry receptor, similar to MERS-CoV. To the best of our knowledge, this is the first report of a bat HKU4r-CoV strain containing a furin protease cleavage site. These findings expand our understanding of coronavirus geographic and host distributions.
- Coronavirus
- , Pangolin HKU4r-CoV
- , Bat HKU4r-CoV
- , Tylonycteris robustula
- , Spillover risk

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10.1016j.virs.2023.11.003-ESM.docx