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Volume 39 Issue 2
April 2024

    . doi: 10.1016/j.virs.2024.02.003
    Citation: Jian Xiao, Xuan Yao, Xuhua Guan, Jinfeng Xiong, Yaohui Fang, Jingyuan Zhang, You Zhang, Abulimiti Moming, Zhengyuan Su, Jiayin Jin, Yingying Ge, Jun Wang, Zhaojun Fan, Shuang Tang, Shu Shen, Fei Deng, . Viromes of Haemaphysalis longicornis reveal different viral abundance and diversity in free and engorged ticks .VIROLOGICA SINICA, 2024, 39(2) : 194-204.  http://dx.doi.org/10.1016/j.virs.2024.02.003
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    长角硬蜱病毒组研究揭示游离蜱和吸血蜱具有不同的病毒丰度与多样性

    • a 中国科学院武汉病毒研究所, 病毒学国家重点实验室与国家病毒资源库, 湖北, 武汉 430071, 中国;

    • b 湖北省疾病预防控制中心, 武汉, 430070, 中国;

    • c 中国科学院大学, 北京, 101408, 中国;

    • d 湖北江夏实验室, 武汉, 430200, 中国 & 海南医学院第二附属医院医学检验科, 海口, 57000, 中国

    • 在东亚常见的长角血蜱可以传播多种致病病毒，包括严重急性发热伴血小板减少综合症病毒（SFTSV）。本研究调查了2019年至2020年间在湖北省三个山区内吸血和未吸血的长角血蜱的病毒组。测序分析鉴定出与参考病毒相关的39个病毒序列。这些病毒序列属于未分类病毒和七个病毒家族：Chuviridae、Nairoviridae、Orthomyxoviridae、Parvoviridae、Phenuiviridae、Rhabdoviridae和Totiviridae。通过生物信息学分析，我们探讨了影响蜱虫携带的病毒组成结构的关键因素。此外，基于全基因组序列的系统发育分析阐明了Henan tick virus（HNTV）、Dabieshan tick virus（DBSTV）、Okutama tick virus（OKTV）和Jingmen tick virus（JMTV）的分子进化特征。基于单只蜱的精细分子流行病学调查表明DBSTV是长角血蜱中最常见的病毒，流行率为12.59%，其次是HNTV（0.35%），但未检测到JMTV和OKTV。这些结果加深了对中国中部地区长角血蜱病毒组的了解，提示蜱虫吸血状态和地理位置对蜱病毒组结构组成方面的影响作用。对本研究中新发现的病毒株，将在后续的调查和研究中加强对这些病毒的监测，全面评估其溢出潜力和对公共卫生的潜在影响。

    Viromes of Haemaphysalis longicornis reveal different viral abundance and diversity in free and engorged ticks

    • a. Key Laboratory of Virology and Biosafety and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;

    • b. Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430070, China;

    • c. University of Chinese Academy of Sciences, Beijing, 101408, China;

    • d. Hubei Jiangxia Laboratory, Wuhan, 430200, China;

    • e. Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830002, China;

    • f. Current address: Department of Medical Laboratory, The Second Affiliated Hospital, Hainan Medical University, Haikou, 57000, China

    • Haemaphysalis longicornis ticks, commonly found in East Asia, can transmit various pathogenic viruses, including the severe fever with thrombocytopenia syndrome virus (SFTSV) that has caused febrile diseases among humans in Hubei Province. However, understanding of the viromes of H. longicornis was limited, and the prevalence of viruses among H. longicornis ticks in Hubei was not well clarified. This study investigates the viromes of both engorged (fed) and free (unfed) H. longicornis ticks across three mountainous regions in Hubei Province from 2019 to 2020. RNA-sequencing analysis identified viral sequences that were related to 39 reference viruses belonging to unclassified viruses and seven RNA viral families, namely Chuviridae, Nairoviridae, Orthomyxoviridae, Parvoviridae, Phenuiviridae, Rhabdoviridae, and Totiviridae. Viral abundance and diversity in these ticks were analysed, and phylogenetic characteristics of the Henan tick virus (HNTV), Dabieshan tick virus (DBSTV), Okutama tick virus (OKTV), and Jingmen tick virus (JMTV) were elucidated based on their full genomic sequences. Prevalence analysis demonstrated that DBSTV was the most common virus found in individual H. longicornis ticks (12.59%), followed by HNTV (0.35%), whereas JMTV and OKTV were not detected. These results improve our understanding of H. longicornis tick viromes in central China and highlight the role of tick feeding status and geography in shaping the viral community. The findings of new viral strains and their potential impact on public health raise the need to strengthen surveillance efforts for comprehensively assessing their spillover potentials.
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      Viromes of Haemaphysalis longicornis reveal different viral abundance and diversity in free and engorged ticks

        Corresponding author: Shu Shen, shenshu@wh.iov.cn
        Corresponding author: Fei Deng, df@wh.iov.cn
      • a. Key Laboratory of Virology and Biosafety and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
      • b. Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430070, China;
      • c. University of Chinese Academy of Sciences, Beijing, 101408, China;
      • d. Hubei Jiangxia Laboratory, Wuhan, 430200, China;
      • e. Xinjiang Key Laboratory of Vector-borne Infectious Diseases, Urumqi, 830002, China;
      • f. Current address: Department of Medical Laboratory, The Second Affiliated Hospital, Hainan Medical University, Haikou, 57000, China
      • Received Date: 09 October 2023
      • Accepted Date: 08 February 2024

      Abstract: Haemaphysalis longicornis ticks, commonly found in East Asia, can transmit various pathogenic viruses, including the severe fever with thrombocytopenia syndrome virus (SFTSV) that has caused febrile diseases among humans in Hubei Province. However, understanding of the viromes of H. longicornis was limited, and the prevalence of viruses among H. longicornis ticks in Hubei was not well clarified. This study investigates the viromes of both engorged (fed) and free (unfed) H. longicornis ticks across three mountainous regions in Hubei Province from 2019 to 2020. RNA-sequencing analysis identified viral sequences that were related to 39 reference viruses belonging to unclassified viruses and seven RNA viral families, namely Chuviridae, Nairoviridae, Orthomyxoviridae, Parvoviridae, Phenuiviridae, Rhabdoviridae, and Totiviridae. Viral abundance and diversity in these ticks were analysed, and phylogenetic characteristics of the Henan tick virus (HNTV), Dabieshan tick virus (DBSTV), Okutama tick virus (OKTV), and Jingmen tick virus (JMTV) were elucidated based on their full genomic sequences. Prevalence analysis demonstrated that DBSTV was the most common virus found in individual H. longicornis ticks (12.59%), followed by HNTV (0.35%), whereas JMTV and OKTV were not detected. These results improve our understanding of H. longicornis tick viromes in central China and highlight the role of tick feeding status and geography in shaping the viral community. The findings of new viral strains and their potential impact on public health raise the need to strengthen surveillance efforts for comprehensively assessing their spillover potentials.

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