. doi: 10.1016/j.virs.2025.07.004
Citation: Xiaoxiao Gao, Cheng Peng, Rongjuan Pei. Thermal tolerance and inactivation of Ebola virus .VIROLOGICA SINICA, 2025, 40(4) : 669-671.  http://dx.doi.org/10.1016/j.virs.2025.07.004

埃博拉病毒的热稳定性与热灭活条件

  • 本研究系统评估了埃博拉病毒(EBOV)在不同温度下的稳定性并验证了EBOV的热灭活条件。实验采用Mayinga毒株,利用TCID50法及盲传实验验证感染性。结果显示,溶液中的EBOV在4 ℃保存7天后仍可检测到感染性(滴度下降1.76log10TCID50/mL),而室温和37 ℃下7天后完全失活; 100 ℃水浴5分钟可彻底灭活病毒, 干浴则需10分钟; 干浴系统中未完全浸没的样本灭活失败率高达90%。研究明确建议采用100 ℃水浴完全浸没≥15分钟作为标准灭活方案,并强调针对不同储存介质中的病毒需通过定量检测验证实验室特异性流程。本研究为BSL-4实验室的EBOV安全操作提供了标准化灭活参数,对高风险病原体的生物安全管理具有重要指导价值。

Thermal tolerance and inactivation of Ebola virus

  • Corresponding author: Rongjuan Pei, rongjuan_pei@wh.iov.cn
  • Received Date: 25 June 2024
    Accepted Date: 07 July 2025
  • Highlights
    1 The investigation demonstrated a high level of tolerance of EBOV to thermal disinfection.
    2 A water-bath is recommended and the tubes should be fully submerged during the process.
    3 The established inactivation guidelines should be followed very strictly.

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    5. Haddock, E., Feldmann, F., Feldmann, H., 2016. Effective chemical inactivation of Ebola virus. Emerg. Infect. Dis. 22, 1292-1294.

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    7. Mitchell, S.W., McCormick, J.B., 1984. Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses. J. Clin. Microbiol. 20, 486-489.

    8. National Health Commission of the People’s Republic of China, 2023. The List of Human Pathogenic Microorganisms. https://zwfw.nhc.gov.cn/kzx/tzgg/gzbxbywswsyhdsp_230/202311/t20231101_2629.html (accesssed 18 August 2023).

    9. Negovetich, N.J., Webster, R.G., 2010. Thermostability of subpopulations of H2N3 influenza virus isolates from mallard ducks. J. Virol. 84, 9367-9376.

    10. Olejnik, J., Leon, J., Michelson, D., Chowdhary, K., Galvan-Pena, S., Benoist, C., Muhlberger, E., Hume, A. J., 2023. Establishment of an inactivation method for Ebola virus and SARS-CoV-2 suitable for downstream sequencing of low cell numbers. Pathogens 12, 342.

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    12. Smither, S.J., Weller, S.A., Phelps, A., Eastaugh, L., Ngugi, S., O'Brien, L.M., Jackie Steward, J., Lonsdale, S.G., Lever, M.S., 2015. Buffer AVL alone does not inactivate Ebola virus in a representative clinical sample type. J. Clin. Microbiol. 53, 3148-3154.

    13. Tuladhar, E., Bouwknegt, M., Zwietering, M.H., Koopmans, M., Duizer, E., 2012. Thermal stability of structurally different viruses with proven or potential relevance to food safety. J. Appl. Microbiol. 112, 1050-1057.

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    Thermal tolerance and inactivation of Ebola virus

      Corresponding author: Rongjuan Pei, rongjuan_pei@wh.iov.cn
    • State Key Laboratory of Virology and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430207, China

    Abstract: Highlights
    1 The investigation demonstrated a high level of tolerance of EBOV to thermal disinfection.
    2 A water-bath is recommended and the tubes should be fully submerged during the process.
    3 The established inactivation guidelines should be followed very strictly.

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