. doi: 10.1016/j.virs.2026.03.003
Citation: Qiran Yin, Han Xiao, Hengrui Hu, Jiang Li, Manli Wang, Zhihong Hu. Development of an HSV-1 F strain BAC system using the synthetic platform .VIROLOGICA SINICA, 2026, 41(2) : 478-481.  http://dx.doi.org/10.1016/j.virs.2026.03.003

利用合成平台开发一种HSV-1 F株的BAC系统

  • I型单纯疱疹病毒(HSV-1)是一种可以作为载体用于开展溶瘤病毒疗法。通过细菌人工染色体(BAC)系统对病毒的基因组进行基因工程改造是可行的,但插入的BAC序列往往会阻碍病毒的复制。在本研究中,我们利用合成生物学构建了一个HSV-1F株BAC平台(F-BAC),其中包含一个两侧具有同向LoxP位点的BAC序列。拯救得到的F-BAC病毒有显著的生长阻碍,GFP表达减少以及噬斑减小的现象。通过体外Cre-LoxP重组技术可以将BAC序列切除以生成F-BAC△病毒,这成功地恢复了病毒的复制动力学,并使细胞病变情况恢复到与亲本病毒相当的水平。这些结果表明我们成功构建了一个功能性的F-BAC平台,并证实合成平台与Cre-loxP介导的重组相结合是一种有效策略,可以产生复制功能完全的HSV-1融合病毒。该系统为开发溶瘤病毒和促进HSV-1基础研究提供了有价值的工具。

Development of an HSV-1 F strain BAC system using the synthetic platform

  • Highlights
    1
    A novel BAC of HSV-1 F-strain was generated by synthetic biology.
    2 The BAC sequence could be removed from F-BAC via in vitro Cre-LoxP recombination.
    3 The resulting F-BACΔ virus shows replication property similar to the wild-type virus.

  • 加载中
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    9. Ling AL, Solomon IH, Landivar AM, Nakashima H, Woods JK, Santos A, Masud N, Fell G, Mo X, Yilmaz AS, Grant J, Zhang A, Bernstock JD, Torio E, Ito H, Liu J, Shono N, Nowicki MO, Triggs D, Halloran P, Piranlioglu R, Soni H, Stopa B, Bi WL, Peruzzi P, Chen E, Malinowski SW, Prabhu MC, Zeng Y, Carlisle A, Rodig SJ, Wen PY, Lee EQ, Nayak L, Chukwueke U, Gonzalez Castro LN, Dumont SD, Batchelor T, Kittelberger K, Tikhonova E, Miheecheva N, Tabakov D, Shin N, Gorbacheva A, Shumskiy A, Frenkel F, Aguilar-Cordova E, Aguilar LK, Krisky D, Wechuck J, Manzanera A, Matheny C, Tak PP, Barone F, Kovarsky D, Tirosh I, Suva ML, Wucherpfennig KW, Ligon K, Reardon DA, Chiocca EA. 2023. Clinical trial links oncolytic immunoactivation to survival in glioblastoma. Nature, 623: 157-166.

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    14. Tanaka M, Kagawa H, Yamanashi Y, Sata T, Kawaguchi Y. 2003. Construction of an excisable bacterial artificial chromosome containing a full-length infectious clone of herpes simplex virus type 1: Viruses reconstituted from the clone exhibit wild-type properties in vitro and in vivo. J Virol, 77: 1382-1391.

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  • 10.1016j.virs.2026.03.003-ESM.doc

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    Development of an HSV-1 F strain BAC system using the synthetic platform

      Corresponding author: Manli Wang, wangml@wh.iov.cn
      Corresponding author: Zhihong Hu, huzh@wh.iov.cn
    • a. State Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China;
    • b. University of Chinese Academy of Sciences, Beijing, 100049, China

    Abstract: Highlights
    1
    A novel BAC of HSV-1 F-strain was generated by synthetic biology.
    2 The BAC sequence could be removed from F-BAC via in vitro Cre-LoxP recombination.
    3 The resulting F-BACΔ virus shows replication property similar to the wild-type virus.

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