SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs

Qian Zhang; Junhua Dong; Pengju Guo; Wenlong Wang; Xueting Zhang; Siyang Pei; Zhaoxu Dong; Ping Qian; Pan Tao

doi:10.1016/j.virs.2026.09.013

October 2026

. doi: 10.1016/j.virs.2026.09.013

Citation: Qian Zhang, Junhua Dong, Pengju Guo, Wenlong Wang, Xueting Zhang, Siyang Pei, Zhaoxu Dong, Ping Qian, Pan Tao. SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs .VIROLOGICA SINICA, 2026, 41(5) : 1200-1212. http://dx.doi.org/10.1016/j.virs.2026.09.013

SwiVirScan：一种用于解析猪相关病毒抗体谱的高通量平台，揭示猪群广泛的病毒暴露史

张谦 ^a,b,c ,
董俊花 ^a,b,c ,
郭鹏举 ^a,b,c ,
王文龙 ^a,b,c ,
张雪婷 ^a,b,c ,
裴思扬 ^a,b,c ,
董兆旭 ^a,b,c ,
钱平 ^a,b,c ,
陶攀 ^a,b,c,,

a. 农业微生物资源发掘与利用全国重点实验室, 华中农业大学, 湖北武汉 430070;
b. 湖北洪山实验室, 湖北武汉 430070;
c. 生猪健康养殖协同创新中心, 华中农业大学动物医学院, 湖北武汉 430070

通讯作者： 陶攀, taopan@mail.hzau.edu.cn
收稿日期： 2026-05-05
录用日期： 2026-09-28

摘要

猪是重要的食物来源、生物医学模型，也是某些人兽共患病毒的储存宿主，但其全面的病毒暴露史仍缺乏系统认识。本研究建立了SwiVirScan，一种基于T7噬菌体展示的高通量血清学平台，包含来源于140种已知猪相关病毒的36,416条肽段。将SwiVirScan应用于127份成年种猪田间血清样本后发现，猪群存在广泛且多样的抗体反应特征：每份样本均识别出来源于多种病毒的肽段，其中包括已报道具有人兽共患意义或潜在跨物种传播能力的病毒。成对分析鉴定出富集的共血清阳性模式，包括PBuV与PCV3之间稳健的关联，并揭示了疫苗靶向病毒血清反应性与其他病毒暴露之间的特异性关联。上述结果表明，SwiVirScan可作为绘制猪群病毒暴露史并优先筛选重点监测与深入研究病毒靶标的高通量平台。
- SwiVirScan
- , 猪相关病毒
- , 抗体谱分析
- , 病毒暴露史
- , 高通量血清学

SwiVirScan, a high-throughput platform for profiling antibodies to swine-associated viruses, reveals extensive viral exposure histories in pigs

Qian Zhang ^a,b,c ,
Junhua Dong ^a,b,c ,
Pengju Guo ^a,b,c ,
Wenlong Wang ^a,b,c ,
Xueting Zhang ^a,b,c ,
Siyang Pei ^a,b,c ,
Zhaoxu Dong ^a,b,c ,
Ping Qian ^a,b,c ,
Pan Tao ^a,b,c,,

a. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China;
b. Hubei Hongshan Lab, Wuhan, 430070, China;
c. Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

Corresponding author: Pan Tao, taopan@mail.hzau.edu.cn
Received Date: 05 May 2026
Accepted Date: 28 September 2026

Abstract

Pigs are crucial as food sources, biomedical models, and reservoirs of some zoonotic viruses, yet their comprehensive viral exposure history remains poorly understood. Here, we developed SwiVirScan, a T7 phage display-based serological platform containing 36,416 peptides derived from 140 known swine-associated viruses. Application of SwiVirScan to 127 field serum samples from adult breeding pigs revealed extensive and diverse antibody signatures: every sample recognized peptides assigned to multiple viruses, including viruses with reported zoonotic relevance or potential for cross-species transmission. Pairwise analysis identified enriched co-seropositivity patterns, including a robust association between porcine bufavirus (PBuV) and porcine circovirus 3 (PCV3), and uncovered distinct associations between seroreactivity to vaccine-targeted viruses and other viral exposures. These findings establish SwiVirScan as a high-throughput platform for mapping viral exposure histories in pig populations and prioritizing viral targets for focused surveillance and investigation.
- SwiVirScan
- , PhIP-seq
- , Serological profiling
- , Swine-associated viruses
- , Vaccine-targeted viruses

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10.1016j.virs.2026.09.013-ESM.docx