Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells

SU Fei; JI Bing; JI Xi-Feng

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August 1999

SU Fei, JI Bing and JI Xi-Feng. Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells[J]. Virologica Sinica, 1999, 14(4): 304-309.

Citation: SU Fei, JI Bing, JI Xi-Feng. Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells .VIROLOGICA SINICA, 1999, 14(4) : 304-309.

一种新的重组腺病毒的构建及其对人肿瘤细胞的影响

1.
武汉大学病毒学研究所,武汉430072

摘要

腺病毒E1A基因诱导细胞凋亡．E1B19K基因及E1B55K基因抑制细胞凋亡，前者被克隆到腺病毒转移载体pCA13的HCMVIE启动子下游．构建成转移载体pCAE1A。采用lipofectin法将PCAE1A和含腺病毒基因组（E1、E3区缺失）的质粒pBHG11共转染293细胞，7～10d后得到重组病毒v5Ad4。用v5Ad4感染人肺腺癌细胞系A549，结果表明v5Ad4有明显杀伤和裂解肿瘤细胞功能。在人胚肺正常二倍体细胞中，v5Ad4没有表现出可见的细胞毒效应。
- 肿瘤基因治疗
- , 腺病毒
- , E1A基因
- , E1B基因

Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells

1.
Institute of Virology，Wuhan University,Wuban 430072

Abstract

E1A gene of adenovirus is an apoptosis-inducing gene and E1B gene of adenovirus is anapoptosis-inhibiting gene. The former one was cloned into adenovirus transfer vector pCA13 under the control of HCMV IE1 promoter, which resulted into transfer vector pCAE1A. PlasmidpCAE1A and pBHG11, which includes almost all the adenovirus genome but E1 region and E3 region were deleted, were cotransfected into 293 cell line. After 7-10 days, recombinant virusv5Ad4(E1B- E3- ) was obtained. v5Ad4 was used to infect A549 cell line. The results showedthat v5Ad4 can kill and lysis tumor cells. In the normal cells, v5Ad4 didn' t show visible cytotoxicity effect.
- Gene therapy for tumor
- , Adenovims
- , E1A gene
- , EIB gene

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Construction of a New Kind of Recombinant Adenovirus and Its effect on Tnmor Cells

1. Institute of Virology，Wuhan University,Wuban 430072

Abstract: E1A gene of adenovirus is an apoptosis-inducing gene and E1B gene of adenovirus is anapoptosis-inhibiting gene. The former one was cloned into adenovirus transfer vector pCA13 under the control of HCMV IE1 promoter, which resulted into transfer vector pCAE1A. PlasmidpCAE1A and pBHG11, which includes almost all the adenovirus genome but E1 region and E3 region were deleted, were cotransfected into 293 cell line. After 7-10 days, recombinant virusv5Ad4(E1B- E3- ) was obtained. v5Ad4 was used to infect A549 cell line. The results showedthat v5Ad4 can kill and lysis tumor cells. In the normal cells, v5Ad4 didn' t show visible cytotoxicity effect.