JIANG Yan, HOU Yu-feng, WANG Kai-min and ZHANG Chang-yin. Detection Kit for the Porcine Circovirus[J]. Virologica Sinica, 2005, 20(1): 89-91.
Citation:
JIANG Yan, HOU Yu-feng, WANG Kai-min, ZHANG Chang-yin.
Detection Kit for the Porcine Circovirus .VIROLOGICA SINICA, 2005, 20(1)
: 89-91.
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摘要
猪圆环病毒(Porcine circovirus, PCV)属圆环病毒科圆环病毒属成员[1]。PCV分两种血清型,即PCV 1 和 PCV 2,其中 PCV 1 由 Tischer 等[2] 于1974年检测到,对猪没有致病性;PCV 2 可引起部分断奶仔猪和育肥猪的断奶仔猪多系统综合征( postweaning multisystemic wasting sy...
关键词:
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PCV2
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PCR
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试剂盒
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特异性
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敏感性
Detection Kit for the Porcine Circovirus
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Corresponding author:
ZHANG Chang-yin,
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Abstract
On the basis of genomic sequence of PCV-2 in the GenBank, two primers for amplification of PCV-2 specified fragments were designed and PCR assay for the detection of PCV-2 was established,the kit was used to detect PCV-2. The kit’s sensitivity, specificity and expiration date were tested. The result suggested the kit had high sensitivity and specificity. It could be stored at least for one year at -20℃.
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References
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Proportional views
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