SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS

CHEN Min-Hui; YAO Xue-Jun; WU Jian-Guo; LIANG Gao-Lin; CHEN Bao-Ping; FANG Ping; LEI Sen-Lin; WANG Qi-Wang; XIAO Tong-Hao

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February 1986

CHEN Min-Hui, YAO Xue-Jun, WU Jian-Guo, LIANG Gao-Lin, CHEN Bao-Ping, FANG Ping, LEI Sen-Lin, WANG Qi-Wang and XIAO Tong-Hao. SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS[J]. Virologica Sinica, 1986, 1(1).

Citation: CHEN Min-Hui, YAO Xue-Jun, WU Jian-Guo, LIANG Gao-Lin, CHEN Bao-Ping, FANG Ping, LEI Sen-Lin, WANG Qi-Wang, XIAO Tong-Hao. SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS .VIROLOGICA SINICA, 1986, 1(1) : 18.

Ⅱ型单纯疱疹病毒感染细胞的扫描与透射电镜对比观察

1.
湖北医学院病毒研究所细胞生物室
2.
武汉部队总医院电镜室
3.
武汉部队总医院电镜室武汉

摘要

以感染复数为3的 HSV—Ⅱ(333株)感染兔婴肾单层细胞,不同间隔时间取材作扫描(SEM)及透射电镜(TEM)观察。感染1小时后,SEM 下显示细胞表面有大量病毒颗粒吸附,颗粒均匀分布或积聚成簇,多数病毒颗粒有囊膜,少数无囊膜。感染2小时后,变化与上基本类似,但有少数细胞变园。感染4小时后,变园的细胞数量增多,在此种细胞的核周区及边缘区积聚有大量病毒颗粒,而核上区则仅见少量散在的病毒颗粒,感染16小时后,部分细胞表面出现大小及形状不规则的孔洞;部分细胞解体成碎片,在孔洞边缘及细胞碎片中,可见有囊膜及无囊膜的病毒颗粒。病毒感染1—2小时后,TEM 下可见有囊膜及无囊膜的病毒颗粒吸附于细胞表面,随后在细胞表面的凹陷及胞浆小泡中,亦可见到类似的病毒颗粒。感染4小时以内的细胞超微结构变化主要有:髓膜的形成,线粒体肿胀,粗面内质网腔扩张及染色质边聚。感染16小时后,细胞呈现严重的变性及解体,在细胞碎片中,亦可见到有囊膜及无囊膜的病毒颗粒。对病毒的吸附,穿入,释放等问题进行了讨论.

SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS

1.
Virus Research Institute
2.
Hubei Medical College
3.
WuhanHuong Shah Hospital lwuhan

Abstract

Baby rabbit kidney cells infected with 333 strain of HSV-2at an MOI of approximately 3 pfu/cell were fixed at sequential intervals.SEM rev- ealed that large amounts of particles,of which a majority and a mino- rity roughly measured 200 nm(enveloped)and 100 nm(naked)respectiv- ely,appeared freely or in cluster on the whole or on a part of cell sur- face membrane 1 hr post infection(p.i.).The feature of particle dis- tribution remained in the surface of cells 2hrs p.i.and4hrs p.i.However, in the central regio...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

SCANNING AND TRANSMISSION ELECTRON MICROSCOPIC OBSERVATION ON HSV-2 INFECTED CELLS

1. Virus Research Institute
2. Hubei Medical College
3. WuhanHuong Shah Hospital lwuhan

Abstract: Baby rabbit kidney cells infected with 333 strain of HSV-2at an MOI of approximately 3 pfu/cell were fixed at sequential intervals.SEM rev- ealed that large amounts of particles,of which a majority and a mino- rity roughly measured 200 nm(enveloped)and 100 nm(naked)respectiv- ely,appeared freely or in cluster on the whole or on a part of cell sur- face membrane 1 hr post infection(p.i.).The feature of particle dis- tribution remained in the surface of cells 2hrs p.i.and4hrs p.i.However, in the central regio...