JI Xi-Peng, HUANG Yong-Xiu, WANG Bing and LIN Sheng-Qing. Expression of Hepatitis B Virus ayw Subtype Genome Cloned in E. coil Cells[J]. Virologica Sinica, 1988, 3(4).
Citation:
JI Xi-Peng, HUANG Yong-Xiu, WANG Bing, LIN Sheng-Qing.
Expression of Hepatitis B Virus ayw Subtype Genome Cloned in E. coil Cells .VIROLOGICA SINICA, 1988, 3(4)
: 328.
乙型肝炎病毒ayw亚型基因在大肠杆菌细胞中的克隆与表达
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1.
武汉大学病毒学系
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2.
湖北老年医学研究所
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3.
湖北医学院附属第一医院 武昌
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摘要
将克隆的乙型肝炎病毒(HBV)ayw亚型基因组DNA插入到质粒pBR322的EcoRI位点,经DNA分子杂交和限制性酶切分析表明,阳性重组体含有HBVDNA,方向为5′末端近BamHI位点,在大肠杆细胞中,插入片段能表达HBcAg和HBeAg及微量的HBsAG。
Expression of Hepatitis B Virus ayw Subtype Genome Cloned in E. coil Cells
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1.
Department of Virology
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2.
Wuhan University
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3.
WuhanFirst Hospital
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Abstract
A cloned Hepatitis B virus (HBV) ayw subtype genome DNA has been inserted into EcoRI site of the plasmid pBR322. The 5 end of the inserted fragment, which was confirmed in positive recombinants by molecular hybridization and restriction digestion closes to BamHI site in the plasmid. We have demonstrated that HBV genome inserted into pB322 is able to express antigens of HBV in E. coli HB101 cells.
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References
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Proportional views
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