OU Yang-Xue-Nong, ZHAO Yong-Qi, NI Da-Dan and SHEN Hong-Kai. Further Studies on HA and HI Test for EHF[J]. Virologica Sinica, 1988, 3(4).
Citation: OU Yang-Xue-Nong, ZHAO Yong-Qi, NI Da-Dan, SHEN Hong-Kai. Further Studies on HA and HI Test for EHF .VIROLOGICA SINICA, 1988, 3(4) : 339.

流行性出血热病毒血凝和血凝抑制试验的进一步研究

  • 本文对流行性出血热研究中传统采用的血凝和血凝抑制试验进行了较大改进。首次用吐温80-乙醚法制备滴度较高的血凝素;以鸽红细胞代替了常用的鹅血球;采用冻存的血球替代了新鲜红细胞。

Further Studies on HA and HI Test for EHF

  • In this paper, we have modified the HA and HI test for EHF. EHFVHAN was made by Tween_(80)-ether method as well as by sucrose-acetone method. Higher HA titer of EHFV-HAN was obtained by Tween_(80)-ether method. The pigeon crythrocytc was most sensitive to the EHFV-HAN among 6 sorts of red blood cells. Frozen pigeon crythrocytes have been used instead of fresh goose erythrocytes.

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    Further Studies on HA and HI Test for EHF

    • 1. Department of Infections Diseases
    • 2. the Second Affiliated Hospital to the Fourth Military Medical College
    • 3. Xi an

    Abstract: In this paper, we have modified the HA and HI test for EHF. EHFVHAN was made by Tween_(80)-ether method as well as by sucrose-acetone method. Higher HA titer of EHFV-HAN was obtained by Tween_(80)-ether method. The pigeon crythrocytc was most sensitive to the EHFV-HAN among 6 sorts of red blood cells. Frozen pigeon crythrocytes have been used instead of fresh goose erythrocytes.

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