Inhibitory Effect of IFN-α and IFN-γ on the Persistent Infection of HSV-Ⅱ

YAO Kun; ZHOU Yao-Xi; TUN Xiao-Ling; ZHOU Feng; CHENG Bao-Geng; TANG Zhu-Ping

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August 1988

YAO Kun, ZHOU Yao-Xi, TUN Xiao-Ling, ZHOU Feng, CHENG Bao-Geng and TANG Zhu-Ping. Inhibitory Effect of IFN-α and IFN-γ on the Persistent Infection of HSV-Ⅱ[J]. Virologica Sinica, 1988, 3(4).

Citation: YAO Kun, ZHOU Yao-Xi, TUN Xiao-Ling, ZHOU Feng, CHENG Bao-Geng, TANG Zhu-Ping. Inhibitory Effect of IFN-α and IFN-γ on the Persistent Infection of HSV-Ⅱ .VIROLOGICA SINICA, 1988, 3(4) : 343.

α.γ干扰素在HSV-Ⅱ持续感染中的抑毒效应

1.
南京医学院微生物教研室
2.
南京医学院电镜室
3.
南京医学院电镜室南京

摘要

用HSV-Ⅱ感染K562细胞系,以后连续传代,培养上清液传至2—3代检测不出感染力,4代以后感染力在10~3TCD_(50)。连传至12代共74天,不仅在K562细胞上建立了HSV-Ⅱ的持续感染模型,而且观察到α.γ干扰素和IL_2粗制品对这种持续感染的抑制效应。通过上清液感染力检测,电镜观察、死活细胞计数、病毒包涵体检出,初步得到如下结论:大剂量α.γ干扰素可完全抑制持续感染的建立,不产生有感染性病毒颗粒。而IL_2的粗制品能减低K562的感染力。但不能完全抑制病毒复制。

Inhibitory Effect of IFN-α and IFN-γ on the Persistent Infection of HSV-Ⅱ

1.
Faculty of Microbiology of Nanjing Medical College
2.
Nanjing

Abstract

K_(562) cells were challenged with HSV-Ⅱ virus and then cultured for successive reproduetion. In the supernatants of the culture no virus was tested in the 2nd and 3nd generations, but in the 4th generation the virus titer was found to be around 10~3TCD_(50). The culture wes carried on smoothly for 12 generations in 74 days. We observed not only the whole course of the persistent in fecton of HSV-Ⅱ on K _(562) cells, but also the influence of IFN-α,IFN-y and crude interleukin 2 on the persistetn infection. ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Inhibitory Effect of IFN-α and IFN-γ on the Persistent Infection of HSV-Ⅱ

1. Faculty of Microbiology of Nanjing Medical College
2. Nanjing

Abstract: K_(562) cells were challenged with HSV-Ⅱ virus and then cultured for successive reproduetion. In the supernatants of the culture no virus was tested in the 2nd and 3nd generations, but in the 4th generation the virus titer was found to be around 10~3TCD_(50). The culture wes carried on smoothly for 12 generations in 74 days. We observed not only the whole course of the persistent in fecton of HSV-Ⅱ on K _(562) cells, but also the influence of IFN-α,IFN-y and crude interleukin 2 on the persistetn infection. ...