Identification of Eleven Nuclear Polyhedrosis Viruses by Pyrolysis Gas Chromatography

SHU Xiang-Min; TANG Xian-Chun

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August 1988

SHU Xiang-Min and TANG Xian-Chun. Identification of Eleven Nuclear Polyhedrosis Viruses by Pyrolysis Gas Chromatography[J]. Virologica Sinica, 1988, 3(4).

Citation: SHU Xiang-Min, TANG Xian-Chun. Identification of Eleven Nuclear Polyhedrosis Viruses by Pyrolysis Gas Chromatography .VIROLOGICA SINICA, 1988, 3(4) : 347.

11株核型多角体病毒的裂解气相色谱分析

朱湘民 ,
汤显春

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

本文介绍了茶毛虫、蓖麻蚕,棉铃虫、斜纹夜蛾、黑点银纹夜蛾以及美国白蛾等6种昆虫的11株核型多角体病毒裂解色谱图的绘制方法。通过指纹图的分析,可明显地鉴别(区分)相同亚群的不同毒株。11株核型多角体病毒既有峰群的特异性,亦有峰高比值的特异性。指纹图分析结果表明,它们是各不相同的毒株,证明了裂解气相色谱法分析鉴定核型多角体病毒的可行性。

Identification of Eleven Nuclear Polyhedrosis Viruses by Pyrolysis Gas Chromatography

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

A procedure for obtaining pyrochromatograms of 11 NPV from 6 species insect was described. The differences among the virulanees of same subgroup ould be clearly distinguished through analysis of fingerprinting. There is specificity on both the shape of peak of NPV from 11 strains and peak height ratio. The results of fingerprinting showed that they were distinctive individually. Further it was shown that to analyse NPV with PyGC is feasible.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification of Eleven Nuclear Polyhedrosis Viruses by Pyrolysis Gas Chromatography

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: A procedure for obtaining pyrochromatograms of 11 NPV from 6 species insect was described. The differences among the virulanees of same subgroup ould be clearly distinguished through analysis of fingerprinting. There is specificity on both the shape of peak of NPV from 11 strains and peak height ratio. The results of fingerprinting showed that they were distinctive individually. Further it was shown that to analyse NPV with PyGC is feasible.