Production and Some Properties of Porcine Spleen Interferon

LIU Hong; YANG Xue-Lou

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August 1988

LIU Hong and YANG Xue-Lou. Production and Some Properties of Porcine Spleen Interferon[J]. Virologica Sinica, 1988, 3(4).

Citation: LIU Hong, YANG Xue-Lou. Production and Some Properties of Porcine Spleen Interferon .VIROLOGICA SINICA, 1988, 3(4) : 389.

猪脾细胞干扰素的制备及其某些特性

刘虹 ,
杨学楼

1.
中国科学院武汉病毒研究
2.
中国科学院武汉病毒研究武汉

摘要

本文报导了猪脾细胞在新城疫病毒-F株(NDV-F)诱生下,能产生抗病毒物质。此物质具有对胰旦白酶敏感,不能通过透析膜等干扰素性质。粗制猪脾细胞干扰素(PSIFN)在乳猪肾原代细胞上的平均效价为1.3×10~5U/ml。用猪干扰素预处理细胞,再加诱生剂,可提高PSIFN产量。PSIFN对酸(pH2.5),加热(56℃),表面活性剂(0.1％SDS)处理均敏感。比较了PSIFN在几种异种细胞上的抗病毒活性,结果表明:在人胚肺细胞上其效价为2.24×10~6u/ml,这比在猪肾细胞上测得的效价高十几倍。在兔肾和小鼠细胞上效价分别为3.2×10~3u/ml和1.15×10~3u/ml。上述结果表明猪脾脏可作为生产高效价外源性干扰素的良好细胞来源。

Production and Some Properties of Porcine Spleen Interferon

LIU Hong ,
YANG Xue-Lou

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

Porcine spleen cells induced with Newcastle disease virus F strain could produce an antiviral substance. The substance was sensitive to trypsin and did not penetrate the dialyser. These indicated that it was characteristic of an Interferon. The antiviral activity of crude porcine spleen cell interferon(PSIFN)was 1.3×10~5u/ml on porket kidney cells. PSIFN production was significantly enhanced by pretreating cells with porcine IFN. The crude PSIFN was less stable to low pH treatment, to 56℃ and to 0.1％ SDS. I...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Production and Some Properties of Porcine Spleen Interferon

LIU Hong ,
YANG Xue-Lou

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: Porcine spleen cells induced with Newcastle disease virus F strain could produce an antiviral substance. The substance was sensitive to trypsin and did not penetrate the dialyser. These indicated that it was characteristic of an Interferon. The antiviral activity of crude porcine spleen cell interferon(PSIFN)was 1.3×10~5u/ml on porket kidney cells. PSIFN production was significantly enhanced by pretreating cells with porcine IFN. The crude PSIFN was less stable to low pH treatment, to 56℃ and to 0.1％ SDS. I...