Morphologic Observations on the Coronavirus-like Particles of Several Animals in the lon-exchange Capture Technique by Electron Microscopy

LI Cheng

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August 1988

LI Cheng. Morphologic Observations on the Coronavirus-like Particles of Several Animals in the lon-exchange Capture Technique by Electron Microscopy[J]. Virologica Sinica, 1988, 3(4).

Citation: LI Cheng. Morphologic Observations on the Coronavirus-like Particles of Several Animals in the lon-exchange Capture Technique by Electron Microscopy .VIROLOGICA SINICA, 1988, 3(4) : 397.

应用离子交换捕捉电镜技术对几种动物类冠病毒形态的观察

李成

1.
中国农科院哈尔滨兽医研究所哈尔滨

摘要

我们应用离子交换捕捉电镜技术对貉子、猪、牛的腹泻粪便作了观察,发现了形态学上与资料所描述的马、牛、猫和人的类冠病毒相同的病毒。离子交换捕捉电镜技术,是用磷酸氢钙(CaHPO_4)通过静电引力将悬浮样品中的蛋白颗粒吸附下来,再用溶解剂将CaHPO_4溶解,使蛋白颗粒游离出来。其具体方法大致分以下几个步骤:一、试剂的制备,(一)CaHPO_4的制备,将O.3mol/L氯化钙(11.1gCaCI_2?H_2O溶解在200ml蒸馏水)和O.3tool/L磷酸氢二钠)14.2gNa_2HPO_4溶解在200ml...