SUN Chang-Zhu, TU Gui-Lan, WANG Ti-Jiu, ZHANG Dai-Min and LI Zhi-Xin. A Rapid Detection of Antigen and Antibody of Adult Diarrhea Rotavirus(ADRV)[J]. Virologica Sinica, 1989, 4(1).
Citation:
SUN Chang-Zhu, TU Gui-Lan, WANG Ti-Jiu, ZHANG Dai-Min, LI Zhi-Xin.
A Rapid Detection of Antigen and Antibody of Adult Diarrhea Rotavirus(ADRV) .VIROLOGICA SINICA, 1989, 4(1)
: 45.
快速检测成人腹泻轮状病毒(ADRV)抗原和抗体的研究
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1.
济南军区军事医学研究所
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陆军54898医院
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3.
陆军146医院 济南
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摘要
本文报道应用反相间接血凝和间接血凝试验技术快速检测ADRV抗原和抗体,一般在60分钟左右作出诊断。用ELISA方法和协同凝集试验作对照,结果一致。本实验与其它肠道病毒不发生交叉凝集反应,与菌痢、肠炎病人粪便和正常人粪便标本也不发生非特异性反应。实验准确可靠。本实验用间接血凝试验技术快速检测了ADRV抗体,观察了流行性腹泻病人血清中ADRV抗体持续时间。发现流行性腹泻患者发病后ADRV抗体存在半年以上,八个月有不同程度的下降,流行地区的健康人的ADRV抗体水平占33％左右,非流行地区正常人的ADRV抗体占11.3％左右。并用血凝抑制试验检测ADRV抗体,与小儿轮状病毒抗体无交叉反应。本试验对流行性腹泻的流行病学研究和临床诊断具有实用意义。
A Rapid Detection of Antigen and Antibody of Adult Diarrhea Rotavirus(ADRV)
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Abstract
Indirect hemagglutination assay, enzyme-linked immunosorbent assay and co-agglutination test were applied to determine antigen and antibody of ADRV, the results of which were the same. Serum Ab to ADRV in patients with epizootic diarrhea persisted for over half a year. The positive rates of the normal human serum Ab to ADRV were 33.3% and 11.3% in the epidemic and non-epidemic areas.
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References
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Proportional views
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