Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484

LIANG Bu-Feng; LIANG Li; JU Shuai-Zhou

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August 1989

LIANG Bu-Feng, LIANG Li and JU Shuai-Zhou. Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484[J]. Virologica Sinica, 1989, 4(4).

Citation: LIANG Bu-Feng, LIANG Li, JU Shuai-Zhou. Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484 .VIROLOGICA SINICA, 1989, 4(4) : 402.

油桐尺蠖卵巢细胞系BS-484克隆株特性的研究

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

通过有限稀释法由Bs—484细胞系中成功地分离出四个克隆株(Bs—484B,E,F,G)。克隆细胞侏的生长特性不同于原细胞株Bs—484,各克隆株之间的形态特征、细胞倍增时间、以及在维持油桐尺蠖核型多角体病毒的复制能力等方面均有差异。用三种同工酶(乳酸脱氢酶、苹果酸脱氢酶和酯酶)比较了各克隆株与原细胞株之间的异同。
- 昆虫细胞系
- , 同工酶
- , 克隆
- , 油桐尺蠖核型多角体病毒

Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484

1.
Wuhan Institute of Virology
2.
Academio Sinica

Abstract

Four clones (Bs-484 B,E, F, G) were successfully isolated from the established cell line of Bs-484 by the method of finite dilution. The growth characteristics of the clonal strains were different from the parent line. The cloned cell strains were different in their predominant morphology, cell doubling times and the relative ability to support replication of the nuclear polyhedrosis virus of Buzura suppressaria (BsNPV). The origin of the cloned cell strains was confirmed by comparing their isozyme profiles...
- Insect cell line
- , Isozyme
- , Clones
- , BsNPV

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Characterization of Clonal Strains from the Buzura suppressaria Ovary Cell Line Bs-484

1. Wuhan Institute of Virology
2. Academio Sinica

Abstract: Four clones (Bs-484 B,E, F, G) were successfully isolated from the established cell line of Bs-484 by the method of finite dilution. The growth characteristics of the clonal strains were different from the parent line. The cloned cell strains were different in their predominant morphology, cell doubling times and the relative ability to support replication of the nuclear polyhedrosis virus of Buzura suppressaria (BsNPV). The origin of the cloned cell strains was confirmed by comparing their isozyme profiles...