FU Chong-Quan, Xiao Yong-Jiu, WU Zhi-Jiang, HOU An-Ceng and XU Xing-Fang. Detection of Specific IgM from Patients with Epidemic Parotitis by Enzyme Linked Immunosorbent Assay[J]. Virologica Sinica, 1990, 5(4).
Citation:
FU Chong-Quan, Xiao Yong-Jiu, WU Zhi-Jiang, HOU An-Ceng, XU Xing-Fang.
Detection of Specific IgM from Patients with Epidemic Parotitis by Enzyme Linked Immunosorbent Assay .VIROLOGICA SINICA, 1990, 5(4)
: 362.
ELISA法检测流行性腮腺炎病毒感染特异性IgM抗体的研究
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武汉市传染病医院病毒室
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武汉市传染病医院病毒室 武汉
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摘要
用流行性腮腺炎(流腮)病毒Enders株接种鸡胚尿囊腔培养,尿囊液经聚乙二醇6000处理制备流腮病毒抗原,用ELISA法检测流腮患者血清中特异性IgM抗体,其敏感性,特异性、重复性和稳定性都很高。 79份流腮患者血清,检出特异性IgM72份,阳性率为91％,32例非流腮患者IgM全部阴性、两者有极显著差异(P<0.01)。 10份血清作血清倍比稀释至1∶3200测IgM仍全部阳性,1∶6400稀释仅1例阴性,1∶12800稀释5例中仍有2例阳性。 10份血清作流腮抗原特异性抗体阻断试验,光密度抑制率均大于50％,平均为87％,10份标本作2-ME和SPA阻断后检测IgM抗体,结果2-ME阻断标本全部阴转,而SPA阻断标本仍阳性,证实所检测为流腮特异性抗体。 24份标本2次重复检测流腮IgM,其阴、阳性结果一致,这期间抗原放4℃ 1个月,提示抗原的稳定性和方法的重复性都很好。本方法敏感性明显高于血凝抑制试验,其阳性率分别为91％和61％,两者有显著差异。而且所用试剂简单经济,操作简便,快速,适用于临床早期诊断,易于广泛推广应用。
Detection of Specific IgM from Patients with Epidemic Parotitis by Enzyme Linked Immunosorbent Assay
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Abstract
Specific IgM from patients with epidemic parotitis ( EP ) was detected by enzyme linked immunosorbent assay (ELISA). The mumps virus antigen was prepared by using mumps virus Ender s strain. The detected IgM antibody was confirmed by 2-EM destruction. Sensitibity of the assay for mumps virus-IgM was high as showing a titer of 1:12800 in the positive sera from the patients. In clinically diagnosed 79 cases from the patients with epidemic parotitis, 72 cases were IgM positive, and the positive rate was 91%. I...
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