Identification of Baculovirus by Dot Hybridization

TUN Zhong-Xin; CAI Yi-Quan

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Volume 06 Issue 1

February 1991

TUN Zhong-Xin and CAI Yi-Quan. Identification of Baculovirus by Dot Hybridization[J]. Virologica Sinica, 1991, 6(1).

Citation: TUN Zhong-Xin, CAI Yi-Quan. Identification of Baculovirus by Dot Hybridization .VIROLOGICA SINICA, 1991, 6(1) : 49.

分子杂交鉴定杆状病毒

吴中心 ,
蔡宜权

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉 430071河南省农科院烟草研究所许昌

摘要

利用苜蓿银纹夜蛾核型多角体病毒(Autographa californica nuclear polyhedrosis virus,AcNPV) DNA EcoRI-I片段作为同源探针,在35℃条件下,通过Southern-blot hybridization将斜纹夜蛾核型多角体病毒(Prodenia Iiturs nuclear polyhedrosis virus. PlNPV)多角体蛋白基因定位于其HindⅢ-A片段。以PlNPV DNA HindⅢ-A片段和PlNPV DNA HindⅢ-C片段分别制备探针,在不同的杂交条件下,对PINPV,甘兰夜蛾NPV(Mamestra brassicae nuclear polyhedrosis virus,MbNPV)油桐尺蠖NPV(Buzura suppresseria nuclear polyhedrosis virus,BsNPV)进行了鉴定。结果表明:由A片段制备的探针,在不同的杂交条件下,具有相应不同的非特异性,而由C片段制备的探针则具有严格的特异性。
- 多角体蛋白基因
- , 基因探针
- , 鉴定

Identification of Baculovirus by Dot Hybridization

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV DNA HindⅢ-C fragments respectively for the identification of PINPV DNA, MbNPV DNA, BsNPV DNA under different hybridization conditions. The results showed that the probe containing the polyhedrin gene was more extensive...
- Polyhedrin gene Gene probe Identification

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Identification of Baculovirus by Dot Hybridization

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: The polyhedrin gene of Prodenia lituea nuclear polyhedrosis virus(PlNPV) was mapped on the PlNPV DNA HindⅢ-A fragment by Southern-blot hybridization with the AcNPV DNA EeoRI-Ⅰ fragment as probe under 35℃ hybridization conditions. Then, two probes were prepared from PINPV DNA HindⅢ-A and PINPV DNA HindⅢ-C fragments respectively for the identification of PINPV DNA, MbNPV DNA, BsNPV DNA under different hybridization conditions. The results showed that the probe containing the polyhedrin gene was more extensive...